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I4144

Sigma-Aldrich

Interleukin-3 from mouse

IL-3, recombinant, expressed in E. coli, lyophilized powder, suitable for cell culture

Synonym(s):

IL-3, mIL-3, Interleukin-3 from mouse

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About This Item

MDL number:
MFCD00164817
UNSPSC Code:
12352202
NACRES:
NA.77

biological source

mouse

Quality Level

200

recombinant

expressed in E. coli

Assay

≥98% (SDS-PAGE)

form

lyophilized powder

potency

0.01-0.1 ng/mL ED50/EC50

quality

endotoxin tested

mol wt

15.1 kDa

packaging

pkg of 10 and 50 μg

technique(s)

cell culture | mammalian: suitable

impurities

≤1.000 EU/μg

color

white to yellow

UniProt accession no.

P01586

storage temp.

−20°C

Gene Information

mouse ... Il3(16187)

General description

Interleukin-3 is a hematopoietic growth factor, which stimulates basophil development and triggers histamine, IL-4 and IL-6 release. It improves monocyte and macrophage activation and phagocytosis, enhances the secretory function of eosinophils. Interleukin-3 promotes endothelial cell proliferation and migration.

Application

Interleukin-3 from mouse has been used for the determination of 5-lipoxygenase product leukotriene C4 (LTC4) formation. It has also been used as a component of RPMI 1640 medium for cell transfection.

Biochem/physiol Actions

Interleukin-3 (IL-3) is a multifunctional protein, originally called colony forming unit-stimulating activity (CFU-SA), and is produced by activated T lymphocytes. IL-3 supports the formation of multilineage colonies in the early development of multipotent hematopoietic progenitor cells. IL-3 has been shown to induce colony formation of macrophages, neutrophils, mast cells, and megakaryocytes from agar-suspended bone marrow cells. IL-3 also interacts with IL-2 to stimulate growth of T lymphocytes and to induce IgG secretion from activated B cells. Other synonyms for IL-3 include pan-specific hemopoietin, multicolony stimulating factor, mast cell growth factor, burst promoting activity, histamine-producing cell stimulating factor, P cell stimulating factor, and WEHI-3 factor.

Physical form

Lyophilized with 0.5 mg of bovine serum albumin. Sterile filtered through a 0.2 μm filter.

Analysis Note

The biological activity is measured by the dose-dependent stimulation of the proliferation of mouse M-NFS-60 cells.

Storage Class Code

11 - Combustible Solids

WGK

WGK 3

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Personal Protective Equipment

dust mask type N95 (US), Eyeshields, Gloves

Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

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Characterization of phosphopeptide motifs specific for the Src homology 2 domains of signal transducer and activator of transcription 1 (STAT1) and STAT3
Wiederkehr-Adam M, et al.
Test, 278(18), 16117-16128 (2003)
Synthesis and evaluation of benzoxazole derivatives as 5-lipoxygenase inhibitors
Song H, et al.
Bioorganic & Medicinal Chemistry, 18(21), 7580-7585 (2010)
Michèle Wiederkehr-Adam et al.
The Journal of biological chemistry, 278(18), 16117-16128 (2003-02-20)
Signal transducers and activators of transcription (STAT) 1 and STAT3 are activated by overlapping but distinct sets of cytokines. STATs are recruited to the different cytokine receptors through their Src homology (SH) 2 domains that make highly specific interactions with
Interleukin-3-deficient mice have increased resistance to blood-stage malaria.
Auclair SR et al
Infection and Immunity, 82(3), 1308-1314 (2014)
Nadja Bakocevic et al.
European journal of immunology, 44(6), 1823-1834 (2014-03-13)
Basophils, a rare leukocyte population in peripheral circulation, are conventionally identified as CD45(int) CD49b(+) FcεRI(+) cells. Here, we show that basophils from blood and several organs of naïve wild-type mice express CD41, the α subunit of α(IIb)β₃ integrin. CD41 expression
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Interleukin Family (IL)

The Interleukins comprise a disparate group of cytokines and growth factors that are produced by and released from leukocytes. Interleukin-1β (1L-1β) is released primarily from stimulated macrophages and monocytes and plays a key role in inflammatory and immune responses and may induce anti-tumor immunity.

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