Skip to Content
Merck
All Photos(1)

Key Documents

H9950

Sigma-Aldrich

Hanahan′s Broth (SOB Medium)

Liquid microbial growth medium

Sign Into View Organizational & Contract Pricing
All Photos(1)

About This Item

UNSPSC Code:
12352200

grade

for molecular biology

sterility

sterile; 0.2 μm filtered

form

liquid

suitability

suitable for nonselective for Escherichia coli and Coliforms

storage temp.

room temp

General description

Hanahan′s (SOB) media is a highly-referenced microbial growth medium used for the cultivation of E. coli. This nutrient-rich microbial broth contains peptides, amino acids, water-soluble vitamins, and carbohydrates.

Application

Suitable for non-selective cultivation of E. coli strains for cloning, DNA plasmid production and production of recombinant proteins. Also suitable for selective cultivation when appropriate antibiotics are added.

Features and Benefits

Hanahan′s (SOB) media provides:
  • Filter-sterilized, ready-to-use format
  • Convenient package size
  • Standard formulation

Components

20 g/L Tryptone
5.0 g/L Yeast Extract
10mM NaCl
2.5mM KCl
10mM MgCl2
10mM MgSO4

Physical form

20 g/L Tryptone
5.0 g/L Yeast Extract
10mM NaCl
2.5mM KCl
10mM MgCl2
10mM MgSO4

Storage Class Code

12 - Non Combustible Liquids

WGK

WGK 3

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

Already Own This Product?

Find documentation for the products that you have recently purchased in the Document Library.

Visit the Document Library

Our team of scientists has experience in all areas of research including Life Science, Material Science, Chemical Synthesis, Chromatography, Analytical and many others.

Contact Technical Service