H0137
HT Media Supplement (50×) Hybri-Max™
lyophilized powder, γ-irradiated, BioXtra, suitable for hybridoma
Synonym(s):
HT Supplement, Hypoxanthine-Thymidin (HT) media supplement
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About This Item
UNSPSC Code:
12352207
Recommended Products
Quality Level
sterility
γ-irradiated
product line
BioXtra
form
lyophilized powder
technique(s)
cell culture | hybridoma: suitable
impurities
endotoxin, tested
storage temp.
−20°C
Related Categories
Application
HT Media supplement is a cocktail hypoxanthine and thymidine qualified for use in hybridoma selection and cell culture applications. HT Media Supplement (50×) Hybri-Max™ has been used as a supplement in INS-1 β-cells co-culture medium. It is suitable for use as medium for hybridoma cells and as Dulbecco′s Modified Eagle Medium (DMEM) for HeLa S3 and MTH1-KO cells.
Reconstitution
Reconstitute contents of vial with 10 ml sterile cell culture medium. Stock solution is sufficient to prepare 500 ml medium. Final working concentration: 100 μM hypoxanthine, 16 μM thymidine.
Legal Information
Hybri-Max is a trademark of Sigma-Aldrich Co. LLC
Storage Class Code
11 - Combustible Solids
WGK
WGK 3
Flash Point(F)
Not applicable
Flash Point(C)
Not applicable
Personal Protective Equipment
dust mask type N95 (US), Eyeshields, Gloves
Certificates of Analysis (COA)
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Protocols
Protocol Guide: XTT Assay for Cell Viability and Proliferation
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