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G5422

Sigma-Aldrich

β-Glycerophosphate disodium salt hydrate

≤1.0 mol % L-α-isomer

Synonym(s):

BGP, Glycerol 2-phosphate disodium salt hydrate

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About This Item

Linear Formula:
(HOCH2)2CHOP(O)(ONa)2 · xH2O
CAS Number:
Molecular Weight:
216.04 (anhydrous basis)
EC Number:
MDL number:
UNSPSC Code:
12352201
PubChem Substance ID:
NACRES:
NA.25

biological source

synthetic

Quality Level

form

powder

impurities

≤0.1% Inorganic phosphorus
≤1.0 mol % L-α-isomer

mp

102-104 °C (lit.)

solubility

water: 50 mg/mL, clear, colorless

SMILES string

O.[Na+].[Na+].OCC(CO)OP([O-])([O-])=O

InChI

1S/C3H9O6P.2Na.H2O/c4-1-3(2-5)9-10(6,7)8;;;/h3-5H,1-2H2,(H2,6,7,8);;;1H2/q;2*+1;/p-2

InChI key

ROPZSVKNEIIIDE-UHFFFAOYSA-L

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Application

β-Glycerophosphate is a classical serine-threonine phosphatase inhibitor used in kinase reaction buffers. BGP is often used in combination with other phosphatase/protease inhibitors for broad spectrum inhibition. It functions as an organic phosphate donor and has been used in culture media for mesenchymal stem cell differentiation to osteoblast-type cells. BGP is also used to buffer M17 media for Lactococcus culture in recombinant protein expression.
β-glycerophosphate is used in homegenization solutions for muscles extracts following fixation.
Initiates differentiation of bone-marrow-derived stem cells embedded in a natural collagen/chitosan gel designed for tissue engineering.

Quality

Low inorganic phosphate content. Mixtures of α- and β-glycerophosphate have historically been used in microbiological media but high levels of inorganic (free) phosphate has been associated with the irregular growth of bacteria and unwanted precipitates.

Pictograms

Exclamation mark

Signal Word

Warning

Hazard Statements

Hazard Classifications

Eye Irrit. 2

Storage Class Code

11 - Combustible Solids

WGK

WGK 3

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Personal Protective Equipment

dust mask type N95 (US), Eyeshields, Gloves

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