product name
HKT-1097, 98061003
biological source
hamster kidney
growth mode
Adherent
karyotype
Modal no. 44
morphology
Fibroblast-like
products
Not specified
receptors
EGF
technique(s)
cell culture | mammalian: suitable
relevant disease(s)
cancer
shipped in
dry ice
storage temp.
−196°C
Cell Line Origin
Syrian Hamster kidney
Cell Line Description
The cell line HKT-1097 has been established from a 14 month old male Syrian Hamster (Mesocricetus auratus) kidney tumour which has been induced by diethylstilbestrol exposure. HKT-1097 cells express epidermal growth factor (EGF) receptor, Erb-B2 and Erb-B3 as well as vimentin, desmin, glial fibrillary acidic protein (GFAP), TGF alpha, PGP 9.5 and S100 protein and neuron-specific enolase. Karyotype analysis revealed a modal number of 44 chromosomes (41% of cells) and polyploidy in approximately 2% of cells. The cells have a doubling time of about 36 hours.
DNA Profile
Not specified
Culture Medium
DMEM + 2 mM Glutamine + 10% Fetal Bovine Serum (FBS).
Subculture Routine
Split sub-confluent cultures (70-80%) 1:4, i.e., seeding at 1 x 10,000 cells / cm2 using 0.25% trypsin or trypsin/EDTA; 5% CO2; 37 °C. Form a multipolar, loosely packed monolayer.
Other Notes
Additional freight & handling charges may be applicable for Asia-Pacific shipments. Please check with your local Customer Service representative for more information.
Certificates of Analysis (COA)
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