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product name
RN 22, 93011414
biological source
rat nerve
growth mode
Adherent
karyotype
n = 43
morphology
Bipolar, stellate
products
S-100 protein
receptors
Not specified
technique(s)
cell culture | mammalian: suitable
relevant disease(s)
cancer
shipped in
dry ice
storage temp.
−196°C
Cell Line Origin
Rat Schwann cell, immature
Cell Line Description
Transplantable tumour of cervical nerve-root induced transplacentally by ethylnitrosourea in an inbred BD-IX rat.
Application
Analysis of Schwann cell development
Culture Medium
DMEM or Ham′s F12 + 2mM Glutamine + 10% Foetal Bovine Serum (FBS).
Subculture Routine
Split sub-confluent cultures (70-80%) 1:5 i.e. seeding at 2-4x10,000 cells/cm2 using 0.25% trypsin or trypsin/EDTA; 5% CO2; 37°C.
Other Notes
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Certificates of Analysis (COA)
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