Recommended Products
product name
Detroit 510, 90102531
biological source
human skin
growth mode
Adherent
karyotype
2n = 46
morphology
Fibroblast
products
Not specified
receptors
Not specified
technique(s)
cell culture | mammalian: suitable
shipped in
dry ice
storage temp.
−196°C
Cell Line Origin
Human Caucasian skin Galactosemia
Cell Line Description
Derived from skin tissue of a 9 month old Caucasian female with Galactosemia. Galactose-1-phosphate transferase negative.
Application
Virus studies
DNA Profile
STR-PCR Data: Amelogenin: X
CSF1PO: 11,12
D13S317: 11,12
D16S539: 11,12
D5S818: 10,12
D7S820: 8,12
THO1: 7,9.3
TPOX: 8
vWA: 15,17
CSF1PO: 11,12
D13S317: 11,12
D16S539: 11,12
D5S818: 10,12
D7S820: 8,12
THO1: 7,9.3
TPOX: 8
vWA: 15,17
Culture Medium
EMEM (EBSS) + 2mM Glutamine + 1% Non Essential Amino Acids (NEAA) + 1mM Sodium Pyruvate (NaP) + 0.1% Lactalbumin hydrolysate (LH) + 10%Foetal Bovine Serum (FBS).
Subculture Routine
Split sub-confluent cultures (70-80%) 1:2 to 1:3 i.e. seeding at 3-5x10,000 cells/cm2 using 0.25% trypsin; 5% CO2; 37°C. Finite lifespan, up to 30 population doublings are expected.
Other Notes
Additional freight & handling charges may be applicable for Asia-Pacific shipments. Please check with your local Customer Service representative for more information.
Certificates of Analysis (COA)
Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.
Already Own This Product?
Find documentation for the products that you have recently purchased in the Document Library.
Our team of scientists has experience in all areas of research including Life Science, Material Science, Chemical Synthesis, Chromatography, Analytical and many others.
Contact Technical Service