FHM

88102401, fish colon (posterior), Epithelial-like

• UNSPSC Code: 41106514

Properties

• product name: FHM, 88102401
• biological source: fish colon (posterior)
• growth mode: Adherent
• karyotype: Modal no. 51
• morphology: Epithelial-like
• products: Not specified
• receptors: Not specified
• technique(s): cell culture | mammalian: suitable
• shipped in: dry ice
• storage temp.: −196°C

Description

Cell Line Origin

Fish Fat Head minnow

Cell Line Description

Derived from tissue posterior to the anus of normal adult minnows. Reported to be capable of growth over a wide temperature range from 4°C-36°C with maximum growth at 34°C. Support growth of the virus of infectious pancreatic necrosis (IPN), cat fish tumour virus (CTV) and Granoff frog virus (FV1). ECHO-II will multiply in FHM but the cytopathic effect is not extensive.

Application

Virus studies: Inf. pacr. necrotic virus, cat fish tumour, and Granoff′s frog viruses

Culture Medium

EMEM (EBSS) + 2mM Glutamine + 1% Non Essential Amino Acids (NEAA) +10% Foetal Bovine Serum (FBS).

Subculture Routine

Split sub-confluent cultures (70-80%) 1:2 to 1:3; 34°C. Dislodge cells by agitation and scraping. Cells are reported to multiply over a wide range of temperatures 0°-36°C, with maximum growth at about 34°C. It is recommended that cultures are grown at 34

Other Notes

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