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biological source
Mouse x Bovine Heterohybridoma
ImmunoDon
Jersey calf lymph node
Myeloma
94A1 (mouse x bovine heteromyeloma)
Reactivity
Application: Fusion partner
technique(s)
cell culture | mammalian: suitable
shipped in
dry ice
storage temp.
−196°C
Cell Line Origin
Mouse 53B3 x Bovine Jersey calf lymph node cells
Cell Line Description
The antibody is directed against sheep red blood cells. It does not react with bovine red cells. This haemocytic antibody is probably directed against the Forssman hapten. This HAT sensitive line can be used as a fusion partner for the production of heterohybridomas.
Immunogen
Sheep red blood cells
Application
Fusion partner
The isotype of the monoclonal antibody produced by this cell line is bovine IgG1.
Culture Medium
RPMI 1640 + 2mM glutamine + 1mM Sodium Pyruvate (NaP) + 10% Foetal Bovine Serum (FBS)
Subculture Routine
Maintain cultures between 3-9x100,000 cells/ml; 5% CO2; 37°C.
Other Notes
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Certificates of Analysis (COA)
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