H357

6092004, human mouth, Polygonal

• UNSPSC Code: 41106514

Properties

• product name: H357, 06092004
• biological source: human mouth
• growth mode: Adherent
• karyotype: Not specified
• morphology: Polygonal
• products: Not specified
• receptors: TGFbeta type I receptor, TGFbeta II receptor, TGFbeta III receptor
• technique(s): cell culture | mammalian: suitable
• relevant disease(s): cancer
• shipped in: dry ice
• storage temp.: −196°C

Description

Cell Line Origin

Human oral squamous cell carcinoma, tongue

Cell Line Description

Established from a squamous cell carcinoma (SCC) of the tongue (20mm) of a 74 year-old male patient. STNMP stage I, well differentiated, node negative tumour. Mutant p53, codon 110 exon 4, G to A; the previously reported mutant Ha-ras status of this cell line: codon: 13, G to A, codon 61, A-G is under investigation. This cell line is highly responsive to TGF (Transforming growth factor )-β and undergoes epithelial to mesenchymal transition. It expresses high levels of TGF-β1. Fahey et al (1996). Tumourigenic in athymic nude mice on subcutaneous injection and non-tumorigenic on orthotopic injection. Haplotype information: A*02,A*31; B*40,B*44; Cw*03,Cw*05

Culture Medium

DMEM:HAMS F12 (1:1) + 2mM Glutamine + 10% Foetal Bovine Serum (FBS) + 0.5 ug/ml sodium hydrocortisone succinate

Subculture Routine

Split sub-confluent cultures (70-80%), approximately every 5-6 days, 1:8 to 1:10 using 0.05% trypsin/EDTA; 5% CO₂; 37°C. Suggested seeding density 5 x 1000 cells/cm². Cells can take approximately 10 minutes to detach, an alternative is to trypsinise 2

Other Notes

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Disclaimer

This cell line has special release conditions: Commercial organisations are required to complete the ′Cell Line Release Authorisation for Research Use in Commercial Organisations′ release conditions form.