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C0549

Sigma-Aldrich

Chloroform:Isoamyl alcohol 24:1

suitable for nucleic acid purification

Synonym(s):

chloroform-isoamyl alcohol

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About This Item

UNSPSC Code:
12190000
NACRES:
NA.52

form

liquid

Quality Level

200

contains

0.6-1.0% ethanol as stabilizer

suitability

suitable for nucleic acid purification

application(s)

agriculture

General description

Chloroform:Isoamyl Alcohol 24:1 is an organic reagent used for DNA extraction and precipitation.

Application

Chloroform:Isoamyl alcohol 24:1 has been used:

  • to extract bacterial genomic DNA, DNA from Daphnia spp, and salmon sperms.
  • for a single DNA precipitation step for high-quality DNA extraction.
  • for protein extraction and RNAse treatment.
  • in phenolic oxidation to separate phenolics from the aqueous portion.
  • for RNA isolation from brown adipose tissue (BAT), inguinal white adipose tissue (iWAT), and epididymal adipose tissue (eWAT).
  • in modified cetyltrimethyl ammonium bromide (CTAB) method to extract total RNA from Impatiens balsamina.

Signal Word

Danger

Hazard Classifications

Acute Tox. 3 Inhalation - Acute Tox. 4 Oral - Carc. 2 - Eye Dam. 1 - Repr. 2 - Skin Irrit. 2 - STOT RE 1 Oral - STOT SE 3

Target Organs

Respiratory system

Storage Class Code

6.1C - Combustible, acute toxic Cat.3 / toxic compounds or compounds which causing chronic effects

WGK

WGK 3

Flash Point(F)

185.0 °F

Flash Point(C)

85 °C

Regulatory Listings

Regulatory Listings are mainly provided for chemical products. Only limited information can be provided here for non-chemical products. No entry means none of the components are listed. It is the user’s obligation to ensure the safe and legal use of the product.

EU REACH Annex XVII (Restriction List)

CAS No.

Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

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Optimisation of DNA extraction from the crustacean Daphnia.
Athanasio CG
PeerJ, 4, e2004-e2004 (2016)
Harnessing microbially generated power on the seafloor.
Tender LM
Nature Biotechnology, 20(8), 821-825 (2002)
Malte Thodberg et al.
Nucleic acids research, 47(4), 1671-1691 (2018-12-20)
Fission yeast, Schizosaccharomyces pombe, is an attractive model organism for transcriptional and chromatin biology research. Such research is contingent on accurate annotation of transcription start sites (TSSs). However, comprehensive genome-wide maps of TSSs and their usage across commonly applied laboratory
A fragrant neighborhood: volatile mediated bacterial interactions in soil.
Schulz-Bohm K
Frontiers in Microbiology, 6, 1212-1212 (2015)
Ning Jia et al.
Molecular cell, 75(5), 944-956 (2019-07-22)
Type III-A CRISPR-Cas surveillance complexes containing multi-subunit Csm effector, guide, and target RNAs exhibit multiple activities, including formation of cyclic-oligoadenylates (cAn) from ATP and subsequent cAn-mediated cleavage of single-strand RNA (ssRNA) by the trans-acting Csm6 RNase. Our structure-function studies have focused
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Overview of common techniques and downstream applications for extraction and purification of genomic DNA, plasmid DNA, and total RNA from cells, tissue, blood, viruses, and other sample types.

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