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B6777

5-Bromo-4-chloro-3-indolyl phosphate p-toluidine salt

Name: 5-Bromo-4-chloro-3-indolyl phosphate <I>p</I>-toluidine salt
Brand: SIGMA

BioReagent, for molecular biology, powder, ≥99%

Synonym(s):

5-Bromo-4-chloro-3-indoxyl phosphate p-toluidine salt, BCIP^® p-toluidine salt, X-phosphate p-toluidine salt

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About This Item

Empirical Formula (Hill Notation):

C₈H₆BrClNO₄P · C₇H₉N

CAS Number:

6578-06-9

Molecular Weight:

433.62

EC Number:

229-506-1

MDL number:

MFCD00040636

UNSPSC Code:

12352204

PubChem Substance ID:

24891823

NACRES:

NA.83

grade

for molecular biology

Quality Level

200

product line

BioReagent

Assay

≥99%

form

powder

solubility

DMF: 20 mg/mL
H₂O: insoluble

foreign activity

Protease, none detected

storage temp.

−20°C

SMILES string

Cc1ccc(N)cc1.OP(O)(=O)Oc2c[nH]c3ccc(Br)c(Cl)c23

InChI

1S/C8H6BrClNO4P.C7H9N/c9-4-1-2-5-7(8(4)10)6(3-11-5)15-16(12,13)14;1-6-2-4-7(8)5-3-6/h1-3,11H,(H2,12,13,14);2-5H,8H2,1H3

InChI key

QEIFSLUFHRCVQL-UHFFFAOYSA-N

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Detection Substrates & Enzymes

Application

For the colorimetric detection of alkaline phosphatase-labeled molecules, 5-Bromo-4-chloro-3-indolyl phosphate (BCIP) and Nitro Blue Tetrazolium (NBT) are available. The BCIP/NBT substrate system is versatile and functions in a variety of applications, including Northern Southern, and Western blotting, in situ hybridization, and immunohistochemistry.
BCIP is provided in two salt forms: the disodium salt which is soluble in water and the p-toluidine form which is soluble in dimethylformamide. These salt forms may be used to prepare a stock solution that in combination with NBT and a reaction buffer, form a substrate solution for alkaline phosphatase. This substrate system, when incubated with alkaline phosphatase, produces an insoluble NBT diformazan product that is easily observable with its purple color. For added convenience, a mixture of BCIP and NBT is provided in an easily dissolvable tablet form or as a ready-to-use liquid.

Packaging

Bottomless glass bottle. Contents are inside inserted fused cone.

Substrates

Histochemical substrate for alkaline phosphatase.

Legal Information

BCIP is a registered trademark of Merck KGaA, Darmstadt, Germany

Storage Class Code

11 - Combustible Solids

WGK

WGK 3

Personal Protective Equipment

dust mask type N95 (US), Eyeshields, Gloves

Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

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