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A9555

Sigma-Aldrich

Monoclonal Anti-Aflatoxin B1 (G1, Q1, B2, G2) antibody produced in mouse

clone AT-B1, ascites fluid

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About This Item

MDL number:
UNSPSC Code:
12352203
NACRES:
NA.46

biological source

mouse

Quality Level

conjugate

unconjugated

antibody form

ascites fluid

antibody product type

primary antibodies

clone

AT-B1, monoclonal

contains

15 mM sodium azide

technique(s)

ELISA: 1:20,000 using aflatoxin B1-BSA

isotype

IgG1

shipped in

dry ice

storage temp.

−20°C

target post-translational modification

unmodified

General description

Monoclonal anti-Aflatoxin B (mouse IgG1 isotype) is 1 derived from the hybridoma produced by the fusion of mouse myeloma cells and splenocytes from an immunized mouse.Aflatoxins are a group of naturally occurring fungal toxins (mycotoxins) synthesized by Aspergillus flavus and Aspergillus parasiticus. It is found as contaminants in human and animal food such as corn, peanuts, tree nuts, cottonseed, cereal crops, beans, casssava, milo, sorghum, copra, rice, dried fish and beer.

Immunogen

aflatoxin B -KLH conjugate

Application

Monoclonal Anti-Aflatoxin B1 (G1, Q1, B2, G2) antibody produced in mouse has been used in indirect enzyme linked immunosorbent assay (ELISA) and fluorescence polarization assay.

Biochem/physiol Actions

Aflatoxin B (AFB) and its metabolites is known to act as potent carcinogens, mutagens and teratogens, in addition to its toxicity. Aflatoxins have been implicated in human hepatocellular carcinoma, outbreaks of aflatoxicosis, Rey′s syndrome, chronic hepatitis and increased mortality from infection in animal husbandry.

Target description

Mycotoxins, a group of chemically diverse secondary fungal metabolites, induce a variety of toxic responses in humans and animals when food and feed containing these compounds are ingested. Rapidly quantitating levels of mycotoxin contamination can help reduce exposure to these toxins.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

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Storage Class Code

10 - Combustible liquids

WGK

WGK 3

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable


Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

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Development of a fluorescence polarization assay for the determination of aflatoxins in grains
Nasir MS and Jolley ME
Journal of Agricultural and Food Chemistry, 50(11), 3116-3121 (2002)
Mycotoxins
Bennett JW, et al.
Clinical Microbiology Reviews, 16(3), 497-497 (2003)
Physicochemical and immunological characterization of chitosan-coated bacteriophage nanoparticles for in vivo mycotoxin modeling
de Andrade CYT, et al.
Carbohydrate Polymers, 185, 63-72 (2018)
Robert J Lee et al.
Scientific reports, 6, 33221-33221 (2016-09-15)
Aflatoxins are mycotoxins secreted by Aspergillus flavus, which can colonize the respiratory tract and cause fungal rhinosinusitis or bronchopulmonary aspergillosis. A. flavus is the second leading cause of invasive aspergillosis worldwide. Because many respiratory pathogens secrete toxins to impair mucociliary
Screening of Lactobacillus casei strains for their ability to bind aflatoxin B1
Hernandez MA, et al.
Food And Chemical Toxicology, 47(6), 1064-1068 (2009)

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