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83697

Sigma-Aldrich

Rhodamine 6G

suitable for fluorescence, BioReagent

Synonym(s):

Basic Red 1

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About This Item

Linear Formula:
C28H31N2O3Cl
CAS Number:
989-38-8
Molecular Weight:
479.01
Colour Index Number:
45160
Beilstein:
3900071
EC Number:
213-584-9
MDL number:
MFCD00012665
UNSPSC Code:
12171500
PubChem Substance ID:
57652965
NACRES:
NA.32

product line

BioReagent

Quality Level

200

form

powder

solubility

H2O: soluble
ethanol: soluble

fluorescence

λex 528 nm; λem 551 nm in methanol

suitability

suitable for fluorescence

SMILES string

Cl.CCNc1cc2OC3=CC(=N/CC)\C(C)=CC3=C(c2cc1C)c4ccccc4C(=O)OCC

InChI

1S/C28H30N2O3.ClH/c1-6-29-23-15-25-21(13-17(23)4)27(19-11-9-10-12-20(19)28(31)32-8-3)22-14-18(5)24(30-7-2)16-26(22)33-25;/h9-16,29H,6-8H2,1-5H3;1H/b30-24+;

InChI key

VYXSBFYARXAAKO-WTKGSRSZSA-N

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Application

Rhodamine 6G has been used as a leukocyte marker to study the interaction between leukocytes and the vascular endothelium. It has also been used to label platelets.

Biochem/physiol Actions

A rhodamine analog useful in Pgp efflux assays. Has been used to characterize kinetics of MRP1- mediated efflux.
Rhodamine 6G is a rhodamine analog useful in P-glycoprotein (Pgp) efflux assays. It has been used to characterize kinetics of multidrug resistance protein 1 (MRP1)-mediated efflux.

Analysis Note

λmax. ∼530 nm, E(1%/1cm) ∼2000-2300 (96% Ethanol)

Signal Word

Danger

Hazard Statements

H301,H317,H318,H410

Hazard Classifications

Acute Tox. 3 Oral - Aquatic Acute 1 - Aquatic Chronic 1 - Eye Dam. 1 - Skin Sens. 1

Storage Class Code

6.1C - Combustible acute toxic Cat.3 / toxic compounds or compounds which causing chronic effects

WGK

WGK 3

Personal Protective Equipment

dust mask type N95 (US), Eyeshields, Gloves

Regulatory Listings

Regulatory Listings are mainly provided for chemical products. Only limited information can be provided here for non-chemical products. No entry means none of the components are listed. It is the user’s obligation to ensure the safe and legal use of the product.

EU REACH Annex XVII (Restriction List)

CAS No.

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Certificates of Analysis (COA)

Lot/Batch Number
BCCH0007
BCCF1630
BCBZ8781
BCBV3573
BCBQ2301V

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T P Santisakultarm et al.
Journal of thrombosis and haemostasis : JTH, 12(12), 2120-2130 (2014-09-30)
Essential thrombocythemia (ET) and polycythemia vera (PV) are myeloproliferative neoplasms (MPNs) that share the JAK2(V617F) mutation in hematopoietic stem cells, leading to excessive production of predominantly platelets in ET, and predominantly red blood cells (RBCs) in PV. The major cause
MinKwan Kim et al.
Scientific reports, 5, 16525-16525 (2015-11-18)
Superresolution fluorescence microscopy possesses an important role for the study of processes in biological cells with subdiffraction resolution. Recently, superresolution methods employing the emission properties of fluorophores have rapidly evolved due to their technical simplicity and direct applicability to existing
Bo Hu et al.
Biosensors & bioelectronics, 49, 499-505 (2013-07-03)
The detection of mercury in biological systems and its imaging is of highly importance. In this work, a ratiometric fluorescence sensor is developed based on fluorescence resonance energy transfer (FRET) with N-acetyl-L-cysteine functionalized quantum dots (NAC-QDs) as donor and Rhodamine
Brendan L Darby et al.
Journal of the American Chemical Society, 136(31), 10965-10973 (2014-07-22)
This study highlights a crucial but often overlooked consideration during sample preparation involving surface-adsorbing species: the competition between analyte adsorption and analyte diffusion/mixing strongly affects the distribution of analytes throughout the sample. In cases of fast analyte adsorption, we argue
Asmahan Abu-Arish et al.
Biophysical journal, 97(8), 2164-2178 (2009-10-22)
The GTPase Ran is a key regulator of molecular transport through nuclear pore complex (NPC) channels. To analyze the role of Ran in its nuclear transport function, we used several quantitative fluorescence techniques to follow the distribution and dynamics of
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