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49525

Sigma-Aldrich

L-Glutamic acid γ-(3-carboxy-4-nitroanilide) ammonium salt

≥99.0% (TLC)

Synonym(s):

L-Glutamic acid 5-(3-carboxy-4-nitroanilide) ammonium salt

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About This Item

Linear Formula:
C12H12N3O7NH4
CAS Number:
Molecular Weight:
328.28
EC Number:
MDL number:
UNSPSC Code:
12352204
PubChem Substance ID:

Quality Level

Assay

≥99.0% (TLC)

form

powder

impurities

≤6% water

solubility

H2O: 100 mg/mL, clear, yellow-green

suitability

suitable as substrate for γ-glutamyltranspeptidase test

storage temp.

2-8°C

SMILES string

N.N[C@@H](CCC(=O)Nc1ccc(c(c1)C(O)=O)[N+]([O-])=O)C(O)=O

InChI

1S/C12H13N3O7.H3N/c13-8(12(19)20)2-4-10(16)14-6-1-3-9(15(21)22)7(5-6)11(17)18;/h1,3,5,8H,2,4,13H2,(H,14,16)(H,17,18)(H,19,20);1H3/t8-;/m0./s1

InChI key

GFABNNMTRVBLPZ-QRPNPIFTSA-N

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Application

L-Glutamic acid γ-(3-carboxy-4-nitroanilide) ammonium salt has been used as a synthetic substrate for gamma-glutamyltransferase (GGT) to determine GGT activity.

Other Notes

Substrate for γ-glutamyl transferase

Storage Class Code

11 - Combustible Solids

WGK

WGK 3

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Personal Protective Equipment

dust mask type N95 (US), Eyeshields, Gloves

Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

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L M Shaw et al.
Clinical chemistry, 23(1), 79-85 (1977-01-01)
The kinetics of human serum gamma-glutamyltransferase (EC 2.3.2.2) were investigated, with use of glycylglycine as a gamma-glutamyl acceptor substrate and gamma-glutamyl-4-nitroanilide and its carboxy derivative, gamma-glutamyl-3-carboxy-4-nitroanilide, as donor substrates. The simultaneous occurrence of both gamma-glutamyltransfer and autotransfer was established by
A kinetic study of gamma-glutamyltransferase (GGT)-mediated S-nitrosoglutathione catabolism.
Angeli V
Archives of Biochemistry and Biophysics, 481(2), 191-196 (2009)

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