3447-020-01
Cultrex 3-D Culture Matrix™ Rat Collagen I
Sign Into View Organizational & Contract Pricing
All Photos(1)
About This Item
UNSPSC Code:
12352200
Recommended Products
biological source
rat
sterility
sterile
mol wt
300 kDa
concentration
5 mg/mL
technique(s)
cell culture | mammalian: suitable
solubility
water: miscible
shipped in
ambient
storage temp.
2-8°C
General description
The 3-D Culture Matrix™ Rat Collagen I may be used as a gel on which to grow cells or a medium additive, alone or in concert with other basement membrane components, to study cellular growth and differentiation in three dimensions in vitro. Type I Collagen is the major structural component of extracellular matrices found in connective tissue and internal organs, but is most prevalent in the dermis, tendons, and bone. It is a 300 kDa molecule composed of two alpha1(I) chains and one alpha2(I) chain that spontaneously forms a triple helix scaffold. This phenomenon can be exploited to promote cell attachment, growth, differentiation, migration, and tissue morphogenesis during development. The 5 mg/ml concentration is very viscous for researchers wanting a thicker Collagen.
Physical form
Solution in 20 mM Acetic Acid, pH 3.4 - 3.6
Other Notes
Functional Assay: 3-D Culture: Collagen I forms a gel when diluted to 0.4 mg/ml at neutral pH and promotes attachment and growth of murine endothelial SVEC4-10 cells. Cell Attachment: Tested for the ability to promote cell attachment and spreading of HT1080 human fibrosarcoma cells.
Sterility Testing: No bacterial or fungal growth detected after incubation at 37°C for 14 days following USP XXIV Chapter 71 sterility test. No mycoplasma contamination detected by PCR.Endotoxin concentrations = 20 EU/ml by LAL assay.
Storage Conditions and product stability: Product is stable for a minimum of 3 months from date of shipment if stored at 4°C. Do Not Freeze.
Legal Information
3-D Culture Matrix is a trademark of Trevigen, Inc.
Storage Class Code
10 - Combustible liquids
WGK
WGK 2
Flash Point(F)
Not applicable
Flash Point(C)
Not applicable
Certificates of Analysis (COA)
Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.
Already Own This Product?
Find documentation for the products that you have recently purchased in the Document Library.
Customers Also Viewed
Claudia Beaurivage et al.
Scientific reports, 10(1), 21475-21475 (2020-12-10)
Inflammatory bowel disease (IBD) is a complex multi-factorial disease for which physiologically relevant in vitro models are lacking. Existing models are often a compromise between biological relevance and scalability. Here, we integrated intestinal epithelial cells (IEC) derived from human intestinal
Our team of scientists has experience in all areas of research including Life Science, Material Science, Chemical Synthesis, Chromatography, Analytical and many others.
Contact Technical Service