NIST2141

Urea

NIST^® SRM^® 2141

• Synonym(s): Carbamide, Carbonyldiamide
• Linear Formula: NH₂CONH₂
• CAS Number: 57-13-6
• Molecular Weight: 60.06
• Beilstein: 635724
• MDL number: MFCD00008022
• UNSPSC Code: 41116107
• PubChem Substance ID: 329818729
• NACRES: NA.24

Properties

• grade: certified reference material
• Quality Level: 100
• form: crystals
• packaging: pkg of 2 g
• manufacturer/tradename: NIST^®
• mp: 132-135 °C (lit.)
• density: 1.335 g/mL at 25 °C (lit.)
• application(s): clinical testing
• SMILES string: NC(N)=O
• InChI: 1S/CH4N2O/c2-1(3)4/h(H4,2,3,4)
• InChI key: XSQUKJJJFZCRTK-UHFFFAOYSA-N

Description

General description

SRM 2141_cert

SRM 2141_SDS

Application

Urea Standard reference material (SRM) is certified for calibrating and standardizing microchemical procedures aimed at determining nitrogen in organic matter. Additionally, it serves as a tool for standardizing techniques for nitrogen determination, when carbon and hydrogen are simultaneously analyzed.

Used for the denaturation of proteins and as a mild solubilization agent for insoluble or denatured proteins. Useful for renaturing proteins from samples already denatured with 6 M guanidine chloride such as inclusion bodies. May be used with guanidine hydrochloride and dithiothreitrol (DTT) in the refolding of denatured proteins into their native or active form.

Features and Benefits

Available with a certificate documentingnitrogen value determined through a modification of the AOAC micro-Kjeldahlmethod.

Other Notes

Example analytes are listed below as a reference. Please download a current certificate at nist.gov/SRM for current analytes and certified values.
Nitrogen (N)

See certificate for values and more details at nist.gov/SRM.

Legal Information

NIST is a registered trademark of National Institute of Standards and Technology

SRM is a registered trademark of National Institute of Standards and Technology

Safety Information

• Storage Class Code: 11 - Combustible Solids
• WGK: WGK 1
• Flash Point(F): Not applicable
• Flash Point(C): Not applicable