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70194

Millipore

Tryptone Water

suitable for microbiology, NutriSelect® Plus

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About This Item

UNSPSC Code:
41171607
NACRES:
NA.74

sterility

non-sterile

Quality Level

200

product line

BioChemika

form

powder

shelf life

limited shelf life, expiry date on the label

composition

sodium chloride, 5.0 g/L
tryptone (tryptic hydrolysate of casein), 10.0 g/L

manufacturer/tradename

NutriSelect® Plus

technique(s)

microbe id | specific enzyme detection: suitable
microbiological culture: suitable

final pH

7.5±0.2 (25 °C)

application(s)

environmental
food and beverages
sample preparation
water monitoring
microbiology

suitability

nonselective for Enterobacter spp.
nonselective for Escherichia coli
nonselective for Proteus spp.
nonselective for Salmonella spp.
nonselective for Staphylococcus spp.
nonselective for bacteria (General Media)
nonselective for coliforms
nonselective for enterobacteriaceae

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Application

A liquid medium for the detection of indole-forming microorganisms due to its high content of tryptophan, it is more reliable than peptone water for this purpose.
Tryptone water is primarily used for the cultivation and maintenance of fastidious aerobic and facultative microorganisms such as Escherichia coli and Pseudomonas species.

Preparation Note

Add 15 g to 1 litre of distilled water. Mix well, distribute into final containers and sterilize by autoclaving at 121°C for 15 minutes.

Other Notes

Direct plating method for enumeration of E. coli in water

Footnote

We offer two media types: the superior granulated GranuCult® and the cost-efficient powdered NutriSelect® culture media, depending on your needs.
The designations basic, plus, or prime are added to indicate the quality control level, from basic quality control to standard QC plus to prime for full regulatory compliance.

Legal Information

GRANUCULT is a registered trademark of Merck KGaA, Darmstadt, Germany
NutriSelect is a registered trademark of Merck KGaA, Darmstadt, Germany

Storage Class Code

11 - Combustible Solids

WGK

WGK 3

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Personal Protective Equipment

dust mask type N95 (US), Eyeshields, Gloves

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Certificates of Analysis (COA)

Lot/Batch Number
BCCG5252
BCCB4467
BCBT2244
BCBR8552V
BCBR5641V

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A H Havelaar et al.
The Journal of applied bacteriology, 64(1), 89-98 (1988-01-01)
The direct plating (DP) method for enumerating Escherichia coli in food was adapted for water analysis by membrane filtration and a standardized protocol was described. The DP method was found to give equal or better recoveries of E. coli than
Handbook of Microbiological Media (2004)

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