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68075

Millipore

C. perfringens Millichrome™ plus Supplement 2

suitable for microbiology

Synonym(s):

C. perfringens chromogenic supplement

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About This Item

UNSPSC Code:
41171614
NACRES:
NA.74

sterility

sterile; 0.45 μm filtered

Quality Level

200

form

dry powder

shelf life

limited shelf life, expiry date on the label

technique(s)

microbiological culture: suitable

solubility

sterile water: 0.12 g/mL

application(s)

food and beverages
pharmaceutical
water monitoring
microbiology

shipped in

ambient

storage temp.

2-8°C

suitability

selective for bacteria: Clostridium perfringens

General description

C. perfringens Millichrome™ plus Supplement 2 is a selective media supplement for the detection and enumeration of C. perfringens. Millichrome™ chromogenic media is a specialized type of culture medium used to detect and isolate specific groups of bacteria based on their ability to produce enzymes with chromogenic substrates. One vial (0.6 g) of the supplement is sufficient for 5L of final media. The prepared supplement is added to the melted C. perfringens Millichrome™ plus Agar Base at 45-50 °C.

Application

C. perfringens Millichrome™ plus Supplement 2 is a selective chromogenic media supplement intended for use in the qualitative detection and enumeration of C. perfringens from various samples.

Packaging

1 vial à 0.6g (with screw cap) is sufficient for 5L

Preparation Note

  • Add 0.12 g of C. perfringens Millichrome® plus supplement 2 in 1 mL of purified water.
  • Filter to sterilize at 0.45 μm
  • Add 1 mL/L of the supplement 2 solution to the melted base at 45/50 °C.

Analysis Note

The Clostridium perfringens is detected by a characteristic orange color after 24 h at an optimal growth temperature of 37°C, while Clostridium difficile forms blue to green colonies after incubation at the same temperature.

Legal Information

CHROMOCULT is a registered trademark of Merck KGaA, Darmstadt, Germany
MilliChrome is a trademark of Merck KGaA, Darmstadt, Germany

Storage Class Code

11 - Combustible Solids

WGK

WGK 3

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