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66304

Millipore

Lysine Decarboxylase Broth

NutriSelect® Plus, suitable for microbiology

Synonym(s):

LD Broth

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About This Item

UNSPSC Code:
41171606
NACRES:
NA.85

Agency

according to ISO 10273:2017

Quality Level

200

sterility

non-sterile

form

powder

shelf life

limited shelf life, expiry date on the label

composition

bromo creol purple, 0.02 g/L
dextrose, 1 g/L
L-lysine hydrochloride, 5 g/L
peptic digest of animal tissue, 5 g/L
yeast extract, 3 g/L

manufacturer/tradename

NutriSelect® Plus

technique(s)

microbe id | specific enzyme detection: suitable
microbiological culture: suitable

final pH

6.8±0.2 (25 °C)

application(s)

clinical testing
environmental
food and beverages
microbiology

suitability

Enterobacter spp.
nonselective and differential for Salmonella spp.
nonselective and differential for enterobacteriaceae

Application

Lysine Decarboxylase Broth is used for distinguishing the Salmonella arizonae from the Bethesda Bellerup Group of Enterobacteriaceae.

Preparation Note

Suspend 14g in 1000ml distilled water. Boil to dissolve the medium completely. Dispense in 5 ml amount into screw capped test tubes. Sterilize by autoclaving at 15 lbs pressure (121°C) for 15 minutes. Cool the tubed medium in an upright position. Inoculate the tubes and overlay with 2-3 ml of sterile mineral oil.

Footnote

We offer two media types: the superior granulated GranuCult® and the cost-efficient powdered NutriSelect® culture media, depending on your needs.
The designations basic, plus, or prime are added to indicate the quality control level, from basic quality control to standard QC plus to prime for full regulatory compliance.

Legal Information

GRANUCULT is a registered trademark of Merck KGaA, Darmstadt, Germany
NutriSelect is a registered trademark of Merck KGaA, Darmstadt, Germany

Storage Class Code

11 - Combustible Solids

WGK

WGK 1

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Personal Protective Equipment

dust mask type N95 (US), Eyeshields, Gloves

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Certificates of Analysis (COA)

Lot/Batch Number
BCCF6768
BCCC1640
BCCB8210
BCBW5464
BCBW2475

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Articles

Differentiation of Escherichia coli from coliforms

Sigma-Aldrich.com presents an article concerning Differentiation of Escherichia coli from coliforms.

Differentiation and Identification Media for Salmonella

Salmonella contamination is the second leading cause of food-borne illness worldwide. Controlling outbreaks of Salmonella is an important task for food regulators, restaurants and the food industry in general. The Salmonella family includes over 2,300 serotypes of bacteria, but two types, Salmonella enteritidis and Salmonella typhimurium, are responsible for about half of all human infections. Most outbreaks of Salmonella are traced back to dairy, poultry and meat products, but Salmonella can grow on nearly any food. Chicken, eggs and their derivative products are particularly high risk.

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