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Key Documents

61768

Supelco

Lectin, immobilized on Agarose CL-4B from Triticum vulgaris

suitable for affinity chromatography

Synonym(s):

Wheat germ agglutinin, immobilized on agarose CL-4B

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About This Item

UNSPSC Code:
12190000
NACRES:
NA.21

technique(s)

affinity chromatography: suitable

Quality Level

100

capacity

0.1-1.0 mg/mL, packed gel capacity (ovomucoid)

storage temp.

2-8°C

Physical form

50% aqueous suspension in 1 M NaCl

Other Notes

Protein isolation from bordetella pertussis that facilitates entry of calmodulin-sensitive adenylate cyclase into animal cells

Storage Class Code

10 - Combustible liquids

WGK

WGK 3

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Personal Protective Equipment

dust mask type N95 (US), Eyeshields, Gloves

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Certificates of Analysis (COA)

Lot/Batch Number
BCCF5609
BCCC4546
BCBZ7229
BCBZ5727
BCBX0189

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Lectin from Phaseolus vulgaris (red kidney bean) Phytohemagglutinin PHA-P, BioXtra, purified by affinity chromatography, lyophilized powder

Sigma-Aldrich

L9017

Lectin from Phaseolus vulgaris (red kidney bean)

M G Donovan et al.
Biochemistry, 28(20), 8124-8129 (1989-10-03)
Bordetella pertussis, the pathogen responsible for whooping cough, releases a soluble calmodulin-sensitive adenylate cyclase into its culture medium. Several investigators have shown that the partially purified adenylate cyclase is capable of entering animal cells and elevating intracellular cAMP levels [Confer

Articles

Affinity Chromatography Troubleshooting

This page shows how to solve practical problems that may occur when running an affinity chromatography column.

Protocols

Performing a Purification of Glycoproteins and Polysaccharides with Agarose Wheat Germ Lectin

This page shows how to purify glycoproteins and polysaccharides by affinity chromatography using Agarose Wheat Germ Lectin from GE Healthcare.

Converting From Linear Fow (cm/hour) to Volumetric Flow Rates (mL/min) and Vice Versa

This page shows how to convert between linear flow and volumetric flow rates in affinity chromatography.

Column Packing and Preparation Protocol for Affinity Chromatography

This page shows how to perform column packing and preparation for affinity chromatography when using Tricorn™ or XK columns available from GE Healthcare.

Sample Preparation for Affinity Chromatography

This page shows how to prepare samples for purification with affinity chromatography.

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