229253
Carminic acid
≥90% (HPLC)
Synonym(s):
Natural Red 4
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About This Item
Empirical Formula (Hill Notation):
C22H20O13
CAS Number:
Molecular Weight:
492.39
Colour Index Number:
75470
Beilstein:
71651
EC Number:
MDL number:
UNSPSC Code:
12171500
PubChem Substance ID:
NACRES:
NA.47
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Quality Level
Assay
≥90% (HPLC)
form
powder
optical activity
[α]20/D +3.1°, c = 1 in H2O
color
purple to red-purple
red to very dark red
mp
136 °C
solubility
1 N acetic acid: 1%, clear to hazy, red
λmax
495 nm
ε (extinction coefficient)
≥13000 at 222-228 nm in ethanol at 0.03 g/L
≥18000 at 275-281 nm in ethanol at 0.03 g/L
application(s)
diagnostic assay manufacturing
hematology
histology
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General description
Carmine is a cochineal product usually furnished as an aluminum and calcium salt of carminic acid. Carminic acid is used for human-medical cell diagnosis and serves the purpose of the histological investigation of sample material of human origin. It is suitable for staining nuclei in histological sections. It is utilized in various staining methods including the procedure for detection of glycogen and mucous substances (Best′s carmine), for nuclear staining, or vital staining.
Application
Carminic acid (CI 75470) is used in Orth′s lithium carmine technique for nuclear staining, Best′s stain for glycogen, and Southgates′s mucicarmine method.
Storage Class Code
11 - Combustible Solids
WGK
WGK 2
Personal Protective Equipment
dust mask type N95 (US), Eyeshields, Gloves
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