CSPD-RO
Roche
CSPD ready-to-use
Disodium 3-(4-methoxyspiro {1,2-dioxetane-3,2′-(5′-chloro)tricyclo [3.3.1.13,7]decan}-4-yl)phenyl phosphate
Synonym(s):
ready-to-use, cspd
About This Item
Recommended Products
Quality Level
Assay
>98% (HPLC)
form
solution
mol wt
Mr 461
packaging
pkg of 1 mL (11655884001[25mM])
pkg of 2 × 50 mL (11755633001[0.25mM] ready-to-use)
manufacturer/tradename
Roche
storage temp.
2-8°C
General description
Application
It may be used for the detection of alkaline phosphatase and alkaline phosphatase conjugates either in solution or on solid supports.
Features and Benefits
- Save time with a fast, highly sensitive substrate.
- Easily strip and reprobe membranes.
- Take multiple exposures of up to two days for each experiment.
- Reuse the substrate by filtering and storing in sodium azide.
Physical form
Preparation Note
Maleic Acid Buffer: 15 to 25 °C
Detection Buffer: 15 to 25 °C
Blocking Stock Solution, 10x: 2 to 8 °C
Blocking Solution: always prepare fresh
Antibody Solution: 2 to 8 °C
Other Notes
Purity: Purified by HPLC
Development time: Variable
Note: First exposure to X-ray film should be 15 to 30 minutes. Examine film and then determine other exposure times based on initial result.
Activated by preincubation (10 minutes at +37°C).
Apply substrate quickly to avoid drying of membrane (background).
Do not use plastic wrap to cover blot; use hybridization bags, acetate sheet protectors, or two sheets of transparent film.
Do not use nitrocellulose membranes.
Repeat exposures can be made several days after addition of substrate.
Storage Class Code
12 - Non Combustible Liquids
WGK
WGK 1
Flash Point(C)
does not flash
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