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Sigma-Aldrich

EmbryoMax® MEF Conditioned Media

EmbryoMax MEF Conditioned Media (MEF-CM) can be used for the feeder-free culture of human ES/iPS cells.

Synonym(s):

MEFCM, MEF-CM, Mouse Embryonic Fibroblast Conditioned Media

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About This Item

UNSPSC Code:
41106514
eCl@ss:
32011203
NACRES:
NA.71

Quality Level

100

manufacturer/tradename

Specialty Media
EmbryoMax®

technique(s)

cell culture | stem cell: suitable

shipped in

ambient

General description

Traditionally, human embryonic stem cell (hESC) or induced pluripotent stem cell (iPSC) culture require mouse embryonic fibroblasts (MEF) support. This requirement makes the protocol complicated and variable. MEF conditioned medium (MEFCM) contains all the growth factors needed in hESC/iPSC culture, eliminating the routine preparation of feeder cells. It is a ready-to-use product for the feeder-free culture of human ES/IPS cells.

Each batch of EmbryoMax MEF Conditioned Media has been tested for hES/iPS cells pluripotency. The media requires an additional 10 ng/ml of bFGF to be added once thawed before use.

Cell Line Description

Primary Mouse Embryo Fibroblasts

Quality

Mycoplasma Testing: PASSED
Bacterial Testing: PASSED
Fungi Testing: PASSED

Storage and Stability

Store at -80C until expiration date on label.

Legal Information

EmbryoMax is a registered trademark of Merck KGaA, Darmstadt, Germany

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Storage Class Code

12 - Non Combustible Liquids

WGK

WGK 1

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

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Protocols

Induced Pluripotent Stem Cell Reprogramming Protocols

Stem cell reprogramming protocols to generate human induced pluripotent stem cells (iPSCs) including viral and non-viral RNA based methods.

Our team of scientists has experience in all areas of research including Life Science, Material Science, Chemical Synthesis, Chromatography, Analytical and many others.

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