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SCM005

Sigma-Aldrich

ReNcell NSC Maintenance Media

ReNcell NSC Maintenance Medium is a defined serum-free, growth factor-free neural stem cell medium that has been optimized for the growth & in vitro differentiation of ReNcell immortalized human neural progenitor cells.

Synonym(s):

ReNcell Neural Stem Cell Media

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About This Item

UNSPSC Code:
12352207
eCl@ss:
32160801
NACRES:
NA.75

Quality Level

form

liquid

manufacturer/tradename

Chemicon®

technique(s)

cell culture | stem cell: suitable

input

sample type neural stem cell(s)

shipped in

dry ice

General description

ReNcell NSC Maintenance Medium is a defined serum-free, growth factor-free medium that has been optimized for the growth and in vitro differentiation of ReNcell immortalized human neural progenitor cells. When used in conjunction with FGF and EGF, the maintenance medium will allow for the proliferation of ReNcell immortalized VM and CX neural stem cells. Withdrawal of the growth factors from ReNcell NSC Maintenance Medium will result in the spontaneous differentiation of ReNcell immortalized neural progenitor cells

Physical form

ReNcell NSC Maintenance Medium contains DMEM/F12, HEPES, L-glutamine, human serum albumin, human transferrin, putrescine dihydrochloride, human recombinant insulin, L-thyroxine, Tri-iodo-thyronine, progesterone, sodium selenite, heparin, corticosterone. The media does not contain antibiotics.

Storage and Stability

SCM005, ReNcell Neural Stem Cell Maintenance Medium should be stored at -20°C until ready to use. Upon thawing, the maintenance medium should be stored at 2-8°C and given a 1-month expiration dating.

Legal Information

CHEMICON is a registered trademark of Merck KGaA, Darmstadt, Germany

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Storage Class Code

12 - Non Combustible Liquids

WGK

WGK 1


Certificates of Analysis (COA)

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A three-dimensional human neural cell culture model of Alzheimer's disease.
Choi, SH; Kim, YH; Hebisch, M; Sliwinski, C; Lee, S; D'Avanzo, C; Chen, H; Hooli et al.
Nature null
A dynamic view of the proteomic landscape during differentiation of ReNcell VM cells, an immortalized human neural progenitor line.
Song, Y; Subramanian, K; Berberich, MJ; Rodriguez, S; Latorre, IJ; Luria, CM; Everley et al.
Scientific data null
Transcriptional signature and memory retention of human-induced pluripotent stem cells.
Marchetto, Maria C N, et al.
Testing, 4, e7076-e7076 (2009)
B J Carroll
Advances in biochemical psychopharmacology, 39, 179-188 (1984-01-01)
The DST is a practical laboratory test that can be used in outpatient or inpatient settings. The required laboratory technology is available at any hospital. No subjective, behavioral, or metabolic side-effects occur with the single-dose administration of dexamethasone, and the

Articles

ReNcell neural progenitors are immortalized human neural stem cell lines that can differentiate into neurons, astrocytes sand oligodendrocytes.

Frequently asked questions about neural stem cells including NSC derivation, expansion and differentiation.

Derivation and characterization of functional human neural stem cell derived oligodendrocyte progenitor cells (OPCs) that efficiently myelinate primary neurons in culture.

Protocols

A stem cell culture protocol to generate 3D NSC models of Alzheimer’s disease using ReNcell human neural stem cell lines.

Step-by-step culture protocols for neural stem cell culture including NSC isolation, expansion, differentiation and characterization.

Our team of scientists has experience in all areas of research including Life Science, Material Science, Chemical Synthesis, Chromatography, Analytical and many others.

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