SAE176-100UG

SAvPhire Monomeric Streptavidin Alpha Fluor 488

• UNSPSC Code: 12352203
• eCl@ss: 32160702

Properties

• conjugate: horse radish peroxidase (HRP)
• Quality Level: 200
• clone: monoclonal
• species reactivity: all
• concentration: (Upon reconstitution in 100 μL of distilled water the concentration will be 1.0 mg/mL. Please refer to guidance on suggested starting dilutions and/or titers per application and sample type.)
• technique(s): flow cytometry: suitable; immunocytochemistry: suitable; western blot: suitable

Description

General description

Streptavidin, a tetrameric protein, has been extensively used in purification and detection of biomolecules. The strong streptavidin-biotin bond can highly suited for attaching various biomolecules to one another or onto a solid support. The streptavidin-biotin complex displays high resistance to organic solvents, denaturants, detergents, and proteolytic enzymes. It can also withstand extremes of temperature and pH. However, one challenge faced by researchers using the streptavidin-biotin system is the target aggregation, which results from multivalent binding. It can cause cross-linking that can interfere with observations at the molecular level. These challenges can be overcome by use of monomeric streptavidin (mSA) that displays structural and binding properties that are suitable for multiple applications based on its small size and monovalent biotin binding. The monomeric form allows for association with biotinylated ligands without inducing aggregation that are observed with multivalent interactions. Monomeric form can also be genetically fused to another protein to form a biotin-binding tag. SAvPhire Monomeric Streptavidin is a is a high-affinity recombinant variant of streptavidin that minimizes the risk of aggregation, precipitation, and crosslinking in pre-incubation steps with biotinylated molecules and offers easier access to the desired targets. Unlike other non-engineered monomeric forms, SAvPhire Monomeric Streptavidin displays highly desirable dissociation kinetics (koff = 63 x 10-3 min-1 for non-engineered monomeric form and 2.1 x 10-3 min-1 for SAvPhire Monomeric Streptavidin) and longer t1/2 (11 min vs 329 min). It is suitable for multiple immunodetection applications. (Ref.: Kroestch, A., et al., (2018). Appl. Microbiol. Biotechnol., 102(23); 10079-10089).

Specificity

SAvPhire Monomeric Streptavidin expressed in E. coli and conjugated with Alpha Fluor 488 that is suitable for immunodetection of biotinylated proteins.

Application

Epitope: Unknown

Quality

Evaluated by Immunocytochemistry in A431 cells.



Immunofluorescent analysis of A431 cells performed using a 1:1 mixture of biotinylated Anti-EGFR (1:1,000 dilution) and SAvPhire Monomeric Streptavidin Alpha Fluor 488 detected EGFR.

Physical properties

15.5 kDa for SAvPhire Monomeric Streptavidin (with an N-terminal His-tag and a C-terminal FLAG tag).

Physical form

SAvPhire Monomeric Streptavidin Alpha Fluor 488 lyophilized from PBS containing D-Mannitol and Sucrose without azide.

Storage and Stability

Stable for 1 year at +2°C to +8°C from date of receipt.

Other Notes

Predicted to react with all species.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Safety Information

• Storage Class Code: 11 - Combustible Solids
• WGK: WGK 3
• Flash Point(F): Not applicable
• Flash Point(C): Not applicable