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MABT201

Sigma-Aldrich

Anti-Integrin alpha5 (Preservative Free) Antibody, clone SNAKA51

clone SNAKA51, from mouse, purified by using protein G

Synonym(s):

Integrin alpha-5, CD49 antigen-like family member E, Fibronectin receptor subunit alpha, Integrin alpha-F, VLA-5, CD49

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About This Item

UNSPSC Code:
12352203
eCl@ss:
32160702
NACRES:
NA.41

biological source

mouse

Quality Level

100

antibody form

purified immunoglobulin

antibody product type

primary antibodies

clone

SNAKA51, monoclonal

purified by

using protein G

species reactivity

human

technique(s)

activity assay: suitable
immunocytochemistry: suitable
western blot: suitable

isotype

IgG2α

NCBI accession no.

NP_001138472

UniProt accession no.

P08648

shipped in

wet ice

target post-translational modification

unmodified

Gene Information

human ... ITGAV(3685)

General description

Integrins are a family of dimeric, transmembrane proteins that mediate cell-cell and extracellular matrix adhesion. Signals transduced by integrins play a role in many biological processes, including cell growth, differentiation, migration and apoptosis. The integrin family is composed of at least 15 alpha and 8 beta subunits that may form over twenty different alpha-beta non-covalently bound dimeric combinations on the cell surface. The alpha subunits all have some homology to each other, as do the beta subunits. Both of the subunits contribute to the binding of the ligand. Integrin alpha subunits contain seven weak sequence repeats in the N-terminal region, which may be important in ligand binding, and have been predicted to fold cooperatively into a single beta-propeller domain with seven beta-sheets. The α5/β1 integrin, also termed VLA5 or CD49e, is identical to the fibronectin receptor and mediates the adhesion of cells to fibronectin.

Immunogen

Whole cells corresponding to human Integrin alpha5.

Application

Activity Assay: A representative lot of this antibody converts alpha5 beta1 integrin into a ligand competant form and promotes fibronectin binding (Clark, K., et al. (2005). J Cell Sci. 118(Pt 2):291-300.).
Immunocytochemistry Analysis: A representative lot of this antibody detected Integin alpha 5 in Primary human foreskin fibroblasts (hFF) (Clark, K., et al. (2005). J Cell Sci. 118(Pt 2):291-300.; Valdembri, D., et al. (2009). PLoS Biol. 7(1):e25.).
Detect Integrin alpha5 (Preservative Free) using this Anti-Integrin alpha5 (Preservative Free) Antibody, clone SNAKA51 validated for use in Western Blotting, Activity Assay, ICC.
Research Category
Cell Structure

Quality

Evaluated by Flow Cytometry in HeLa cell treated with fibronectin.
Flow Cytometry Analysis: 1 µg of this antibody detected Integrin alpha5 in 0.5X10^6 HeLa cells.

Target description

116 kDa calculated

Physical form

Protein G Purified
Purified mouse monoclonal IgG2aκ in buffer containing PBS without preservatives.

Storage and Stability

Stable for 1 year at -20°C from date of receipt.

Analysis Note

Control
HeLa cells

Other Notes

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

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Storage Class Code

12 - Non Combustible Liquids

WGK

WGK 2

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

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A specific alpha5beta1-integrin conformation promotes directional integrin translocation and fibronectin matrix formation.
Clark, Katherine, et al.
Journal of Cell Science, 118, 291-300 (2005)
Bochuan Li et al.
The Journal of clinical investigation, 129(3), 1167-1179 (2019-01-11)
Local flow patterns determine the uneven distribution of atherosclerotic lesions. This research aims to elucidate the mechanism of regulation of nuclear translocation of Yes-associated protein (YAP) under oscillatory shear stress (OSS) in the atheroprone phenotype of endothelial cells (ECs). We
Erica Torchiaro et al.
Oncotarget, 7(1), 712-728 (2015-12-02)
The molecular mechanisms orchestrating peritoneal and hematogenous metastases of ovarian cancer cells are assumed to be distinct. We studied the p90RSK family of serine/threonine kinases that lie downstream the RAS-ERK/MAPK pathway and modulate a variety of cellular processes including cell
Mark S Filla et al.
Investigative ophthalmology & visual science, 60(12), 3897-3913 (2019-09-19)
Fibronectin fibrillogenesis is an integrin-mediated process that may contribute to the pathogenesis of primary open-angle glaucoma (POAG). Here, we examined the effects of αvβ3 integrins on fibrillogenesis in immortalized TM-1 cells and human trabecular meshwork (HTM) cells. TM-1 cells overexpressing
Donatella Valdembri et al.
PLoS biology, 7(1), e25-e25 (2009-01-30)
Neuropilin 1 (Nrp1) is a coreceptor for vascular endothelial growth factor A165 (VEGF-A165, VEGF-A164 in mice) and semaphorin 3A (SEMA3A). Nevertheless, Nrp1 null embryos display vascular defects that differ from those of mice lacking either VEGF-A164 or Sema3A proteins. Furthermore

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