biological source
mouse
Quality Level
conjugate
unconjugated
antibody form
purified antibody
antibody product type
primary antibodies
clone
2G10, monoclonal
mol wt
calculated mol wt 139.51 kDa
observed mol wt ~150 kDa
purified by
using protein G
species reactivity
human
species reactivity (predicted by homology)
hamster
packaging
antibody small pack of 100 μL
technique(s)
western blot: suitable
isotype
IgG1κ
epitope sequence
N-terminal half
Protein ID accession no.
UniProt accession no.
shipped in
2-8°C
target post-translational modification
unmodified
Gene Information
hamster ... SCAP(100689048)
General description
Sterol regulatory element-binding protein cleavage-activating protein (UniProt P97260; also known as SCAP, SREBP cleavage-activating protein) is encoded by the SCAP gene (Gene ID 100689048) in hamster species. SCAP is a multi-pass membrane protein with five cytoplasmic domains, eight transmembrane domains, and four lumenal domains. The C-terminal domain of SCAP mediates association with SREBPs, while transmembrane helices 2 6 comprise the sterol-sensing domain that mediates sterol-induced binding of SCAP to Insigs. SCAP is mainly localized in endoplasmic reticulum and Golgi apparatus. It requires the presence of SPRING for proper localization to endoplasmic reticulum. It mediates the activation of membrane-bound transcription factors known as sterol-regulatory element binding proteins (SREBPs) for transcribing genes encoding cholesterol biosynthetic enzymes. Low sterol concentrations are reported to trigger release of Insig and lead to a conformational change in the SSD domain (aa 284-442) of SCAP, unmasking of the ER export signal and promoting recruitment into COPII-coated vesicles and transport of the SCAP-SREBP to the Golgi. In the Golgi, SREBPs are proteolytically processed releasing the transcription factor fragment of SREBPs from the membrane. At high sterol concentrations, formation of a ternary complex with Insig leads to masking of the ER export signal in SCAP, which promotes retention of the complex in the endoplasmic reticulum. (Ref.: Kober, DL., et al. (2020). Proc. Natl. Acad. Sci. USA. 117(45); 28080-28091).
Specificity
Clone 2G10 is a mouse monoclonal antibody that detects Sterol regulatory element-binding protein cleavage-activating protein (SACP).
Immunogen
Recombinant fragment corresponding to the first 767 amino acids of the Chinese Hamster SCAP protein combined with Ribi adjuvant.
Application
Quality Control Testing
Evaluated by Western Blotting in PC3 cell lysate.
Western Blotting Analysis: A 1:500 dilution of this antibody detected SCAP in PC3 cell lysate.
Tested Applications
Western Blotting Analysis: A representative lot detected SCAP in Western Blotting applications (Kober D.L., et al. (2020). Proc Natl Acad Sci USA. 117(45):28080-28091).
Note: Actual optimal working dilutions must be determined by end user as specimens, and experimental conditions may vary with the end user
Evaluated by Western Blotting in PC3 cell lysate.
Western Blotting Analysis: A 1:500 dilution of this antibody detected SCAP in PC3 cell lysate.
Tested Applications
Western Blotting Analysis: A representative lot detected SCAP in Western Blotting applications (Kober D.L., et al. (2020). Proc Natl Acad Sci USA. 117(45):28080-28091).
Note: Actual optimal working dilutions must be determined by end user as specimens, and experimental conditions may vary with the end user
Anti-SCAP, clone 2G10, Cat. No. MABS2288, is a mouse monoclonal antibody that SREBP cleavage-activating protein (SCAP) and is tested for use in Western Blotting.
Physical form
Purified mouse monoclonal antibody IgG1 in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.
Storage and Stability
Recommend storage at +2°C to +8°C. For long term storage antibodies can be kept at -20°C. Avoid repeated freeze-thaws.
Other Notes
Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.
Disclaimer
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
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Storage Class Code
12 - Non Combustible Liquids
WGK
WGK 1
Flash Point(F)
Not applicable
Flash Point(C)
Not applicable
Certificates of Analysis (COA)
Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.
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