MABN628

Anti-Cox-1, clone 5C5.1 Antibody

clone 5C5.1, 1 mg/mL, from mouse

• Synonym(s): Cytochrome c oxidase subunit 1, Cytochrome c oxidase polypeptide I
• UNSPSC Code: 12352203
• eCl@ss: 32160702
• NACRES: NA.41

Properties

• biological source: mouse
• Quality Level: 100
• antibody form: purified antibody
• antibody product type: primary antibodies
• clone: 5C5.1, monoclonal
• species reactivity: human
• concentration: 1 mg/mL
• technique(s): immunohistochemistry: suitable; western blot: suitable
• isotype: IgG1κ
• NCBI accession no.: YP_003024028
• UniProt accession no.: P00395
• shipped in: wet ice
• target post-translational modification: unmodified
• Gene Information: human ... MT-CO1(4512)

Description

General description

Cox-1, also known as Cytochrome C oxidase subunit 1, Cytochrome C oxidase polypeptide I, and encoded by the gene MT-CO1, CO1, COX1, MTCO1, is the catalytic subunit of Cytochrome C oxidase that forms the final reduction of oxygen to water in the respiratory chain. Subunits 1-3 form the functional core. Electrons originating in Cytochrome C are transferred via the copper A center of subunit 2 and the heme A of subunit 1 to the bimetallic center formed by the heme A3 and Copper B. The Cox-1 gene is encoded in the mitochondrion and the protein localizes to the inner mitochondrion membrane. It is expressed in all cells that contain functional mitochondria and mutations in Cox-1 are associated with various mitochondrial dysfunctional diseases that manifest themselves in defective vision, anemia, cardiomyopathy, muscle weakness and even some forms of cancer.

Immunogen

KLH-conjugated linear peptide corresponding to human Cox-1.

Application

Detect Cox-1 using this Anti-Cox-1 antibody, clone 5C5.1 validated for use in western blotting & IHC.

Immunohistochemistry Analysis: A 1:500-2,000 dilution from a representative lot detected Cox-1 in human thalamus, human kidney and human cerebellum tissue.

Research Category
Neuroscience

Research Sub Category
Developmental Signaling

Quality

Evaluated by Western Blotting in HeLa cell lysate.

Western Blotting Analysis: 1.0 µg/mL of this antibody detected Cox-1 in 10 µg of HeLa cell lysate.

Target description

~65 kDa observed. Uncharacterized band(s) may appear in some lysates.

Physical form

Format: Purified

Protein G Purified

Purified mouse monoclonal IgG1κ in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.

Storage and Stability

Stable for 1 year at 2-8°C from date of receipt.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Safety Information

• Storage Class Code: 12 - Non Combustible Liquids
• WGK: WGK 1
• Flash Point(F): Not applicable
• Flash Point(C): Not applicable