MABF588

Anti-CD45.1 Antibody (mouse), PE, clone A20

clone A20, 0.2 mg/mL, from mouse

• Synonym(s): Receptor-type tyrosine-protein phosphatase C, Leukocyte common antigen, L-CA, Lymphocyte antigen 5, Ly-5, T200, CD45
• UNSPSC Code: 12352203
• eCl@ss: 32160702
• NACRES: NA.41

Properties

• biological source: mouse
• Quality Level: 100
• conjugate: PE
• antibody form: affinity isolated antibody
• antibody product type: primary antibodies
• clone: A20, monoclonal
• species reactivity: mouse
• packaging: antibody small pack of 25 μg
• concentration: 0.2 mg/mL
• technique(s): flow cytometry: suitable; immunohistochemistry: suitable; immunoprecipitation (IP): suitable
• isotype: IgG2aκ
• UniProt accession no.: P06800
• shipped in: wet ice
• target post-translational modification: unmodified
• Gene Information: mouse ... Ptprc(19264)

Description

General description

The A20 antibody reacts with mouse CD45.1, also known as Ly5.1, which is a strain-specific allelic form of the CD45 Leukocyte Common Antigen (LCA). Functionally, CD45 is a protein tyrosine phosphatase whose broad cell distribution supports a critical role in many leukocyte functions, including regulation of signal transduction and cell activation associated with the T cell and B cell receptors. The A20 antibody is typically used as a leukocyte marker in Ly5.1 mouse strains: SJL/J, DA, STS/A and RIII. The antibody has been demonstrated to specific for CD45.1 and is not cross-reactive with CD45.2 bearing cells.

This mouse monoclonal antibody Anti-CD45.1 (mouse), PE, clone A20 validated for use in IP, IHC & Flow Cytometry for the detection of CD45.1.

Quality

The monoclonal antibody was purified from tissue culture supernatant via affinity chromatography. The purified antibody was conjugated under optimal conditions, with unreacted dye removed from the preparation.

Flow Cytometry Analysis: 0.5 µg of this antibody detected CD45.1 in one million C57Bl/6 or SJL splenocytes.

Safety Information

• Storage Class Code: 12 - Non Combustible Liquids
• WGK: nwg
• Flash Point(F): Not applicable
• Flash Point(C): Not applicable