MAB4625

Anti-Bcl-X Antibody, clone 2H12

clone 2H12, Chemicon^®, from mouse

• Synonym(s): Bcl-2-like Protein
• UNSPSC Code: 12352203
• eCl@ss: 32160702
• NACRES: NA.41

Properties

• biological source: mouse
• Quality Level: 100
• antibody form: purified immunoglobulin
• antibody product type: primary antibodies
• clone: 2H12, monoclonal
• species reactivity: rat, pig, mouse, human
• manufacturer/tradename: Chemicon^®
• technique(s): flow cytometry: suitable; immunofluorescence: suitable; immunohistochemistry: suitable; immunoprecipitation (IP): suitable; western blot: suitable
• isotype: IgG2a
• NCBI accession no.: NM_001191.2; NM_138578.1
• UniProt accession no.: Q07817
• shipped in: wet ice
• target post-translational modification: unmodified
• Gene Information: human ... BCL2L1(598)

Description

General description

Bcl-X has two isoforms, Bcl-XL (long), a 241-aa protein, and Bcl-XS (short), a 178-aa protein lacking a 63 aa domain that is well conserved among members of the Bcl-2 family.

Specificity

MAB4625 recognizes a protein of 27 kDa, identified as the Bcl-X protein. Reacts with both Bcl-XS and Bcl-XL.

Immunogen

Synthetic peptide, aa 3-14 of human Bcl-X protein. This amino acid sequence is shared by human and murine Bcl-X protein.

Application

Research Category
Apoptosis & Cancer

Research Sub Category
BCL2 & Inhibition

This Anti-Bcl-X Antibody, clone 2H12 is validated for use in FC, IF, IP, WB, IH for the detection of Bcl-X.

Wstern Blot: 1μg/mL (Hsu, 1997a; Hsu, 1997b)

Flow Cytometry

Immunofluorescence Immunoprecipitation: native protein (2ug/mg protein lysate).

Immunohistochemistry (frozen and paraffin): 2-4ug/mL for 30 minutes at room temperature.

Optimal working dilutions must be determined by end user.

Physical form

Format: Purified

Protein A purified immunoglobulin in 10mM PBS, pH 7.4, with 0.2% BSA and 0.09% sodium azide.

Storage and Stability

Maintain at 2-8°C for up to 12 months.

Other Notes

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

Legal Information

CHEMICON is a registered trademark of Merck KGaA, Darmstadt, Germany

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Safety Information

• Storage Class Code: 12 - Non Combustible Liquids
• WGK: WGK 2
• Flash Point(F): Not applicable
• Flash Point(C): Not applicable