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MAB4416

Sigma-Aldrich

Anti-Plet-1 (Placenta-expressed transcript 1 protein) Antibody, clone 1D4

clone 1D4, from rat

Synonym(s):

chromosome 11 open reading frame 34, hypothetical protein LOC349633, placenta expressed transcript 1

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About This Item

UNSPSC Code:
12352203
eCl@ss:
32160702
NACRES:
NA.41

biological source

rat

Quality Level

antibody form

purified antibody

antibody product type

primary antibodies

clone

1D4, monoclonal

species reactivity

human

species reactivity (predicted by homology)

mouse (based on 100% sequence homology)

technique(s)

flow cytometry: suitable
immunocytochemistry: suitable
immunohistochemistry: suitable
western blot: suitable

isotype

IgG2aκ

NCBI accession no.

UniProt accession no.

shipped in

wet ice

target post-translational modification

unmodified

Gene Information

human ... PLET1(349633)

General description

Placenta-expressed transcript-1 (Plet-1) is a recently identified protein that as of yet has no known function and is not associated with any one family of proteins. Its observation has been restricted to developing mesonephros and pharyngeal endoderm. Observation in adult tissue is more widely expressed, having been found in several locations such as mammary, pancreas and prostate epithelia. Its location in the pancreas is notable as the pancreas is a well known reserve for stem cells and progenitor cells. Within the pancreas, Plet-1 has been shown to be localized in the major duct epithelium, and as such will serve as an excellent marker for epithelial progenitor cells and stem cells, and as a useful implement for observing the genetics of linear relationships and the operation of molecular mechanisms for homeostasis, development and wound healing in organ and tissue systems.
Recently, Plet-1 has been identified as a specific marker of early thymic epithelial progenitor cells. In the postgastrulation embryo, Plet-1 expression is highly restricted to the developing pharyngeal endoderm and mesonephros. This antibody recognizes the same protein as MTS20 and MTS24.

Specificity

This antibody recognizes Plet-1.

Immunogen

Epitope: Unknown
Full length recombinant protein corresponding to Plet-1.

Application

Flow Cytometry Analysis: A previous lot was used by an independent laboratory in FC (Depreter, M.G.L., et al., 2008).

Immunohistochemistry Analysis: A previous lot was used by an independent laboratory in IH (Depreter, M.G.L., et al., 2008).

Immunocytochemistry Analysis: A previous lot was used by an independent laboratory in IC (Depreter, M.G.L., et al., 2008).
Research Category
Stem Cell Research
Research Sub Category
Pluripotent & Early Differentiation
This Anti-Plet-1 (Placenta-expressed transcript 1 protein) Antibody, clone 1D4 is validated for use in WB, IC, IH, FC for the detection of Plet-1 (Placenta-expressed transcript 1 protein).

Quality

Evaluated by Western Blot in human placenta cell lysate.

Western Blot Analysis: A 1:2,500 dilution of this antibody detected Plet-1 in 10 µg of human placenta cell lysate.

Target description

~ 23 kDa

Physical form

Format: Purified
Protein G Purified
Purified rat monoclonal IgG2aκ in buffer containing 0.1 M Tris-Glycine (pH 7.4, 150 mM NaCl) with 0.05% sodium azide.

Storage and Stability

Stable for 1 year at 2-8°C from date of receipt.

Analysis Note

Control
Human placenta cell lysate

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

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Storage Class Code

12 - Non Combustible Liquids

WGK

WGK 1

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable


Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

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