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Key Documents

MAB2035

Sigma-Aldrich

Anti-Aggrecan Antibody

CHEMICON®, mouse monoclonal, MK-302

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About This Item

UNSPSC Code:
12352203
eCl@ss:
32160702
NACRES:
NA.41

product name

Anti-Chondroitin 6 Sulfate Antibody, clone MK-302, ascites fluid, clone MK-302, Chemicon®

biological source

mouse

Quality Level

100

antibody form

ascites fluid

antibody product type

primary antibodies

clone

MK-302, monoclonal

species reactivity

canine, sheep, rat, rabbit, mouse, bovine, human

manufacturer/tradename

Chemicon®

technique(s)

ELISA: suitable
radioimmunoassay: suitable
western blot: suitable

isotype

IgG1

NCBI accession no.

NM_001135.2
NM_013227.2

UniProt accession no.

P16112

shipped in

dry ice

target post-translational modification

unmodified

Gene Information

human ... ACAN(176)

Specificity

Reacts with a wide range of cartilage proteoglycans after either chondroitinase (ABC or AC) or testicular hyaluronidase digestion. Native proteoglycans do not react with MAB2035. Antibody binding to epitope is successfully inhibited by disaccharides of chondroitin-6-sulfate. Recognizes the chondroitin-6-sulfate stubs (preferentially in clusters) of high density cartilage proteoglycans of different species. No cross-reactivity with dermatan sulfate, keratan sulfate or chondroitin-4-sulfate.

Immunogen

Chondroitinase ABC-digested adult human aggrecan.

Application

Anti-Chondroitin 6 Sulfate Antibody, clone MK-302 is an antibody against Chondroitin 6 Sulfate for use in ELISA, RIA & WB.
ELISA at 1:100-1:5,000

RIA at 1:100-1:200.

Chondroitinase ABC digestion prior to antibody reaction is required for antibody reactivity. {0.5U/mL in 100mM Tris-HCL, 30 minutes room temperature} A dramatic proteolytic digestion of the core protein (e.g. with papain or pronase) significantly reduces the antibody reactivity.

Optimal working dilutions must be determined by the end user.

Physical form

Ascites liquid; no preservative.

Legal Information

CHEMICON is a registered trademark of Merck KGaA, Darmstadt, Germany

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Storage Class Code

10 - Combustible liquids

WGK

WGK 1

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

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