MAB1946-IM

Anti-Nidogen 1 Antibody, clone ELM1

clone ELM1, from rat

• Synonym(s): Nidogen-1, NID-1, Entactin
• UNSPSC Code: 12352203
• eCl@ss: 32160702

Properties

• biological source: rat
• Quality Level: 100
• antibody form: purified antibody
• antibody product type: primary antibodies
• clone: ELM1, monoclonal
• species reactivity: mouse
• packaging: antibody small pack of 25 μL
• technique(s): immunocytochemistry: suitable; immunohistochemistry: suitable; western blot: suitable
• isotype: IgG2aκ
• NCBI accession no.: NP_035047
• UniProt accession no.: P10493
• target post-translational modification: unmodified
• Gene Information: mouse ... Nid1(18073)

Description

General description

Nidogen-1 (UniProt: P10493; also known as NID-1, Entactin) is encoded by the Nid1 (also known as Ent) gene (Gene ID: 18073) ins murine species. Nidogen-1 is a highly conserved sulfated monomeric glycoprotein that is widely distributed in basement membranes and tightly associates with laminin. It is post-translationally N- and O-glycosylated. It is synthesized with a 28 aa signal peptide that is subsequently cleaved to produce the mature form. Nidogen -1 contains six EGF-like domains, four LDL-receptor class B repeats, one NIDO domain, one nidogen G2 barrel domain, and one thyroglobulin type 1 domain. Nidogen-1 shares about 50% homology with Nidigen-2 and shares similar adhesive properties. There is also a high degree of homology between murine and human Nidogen-1. Nidogen is expressed during embryonic and fetal development exclusively in fully developed basement membranes of the ectoderm and is not expressed in the developing endodermal basement membrane or in membranes disrupted during mesoderm formation.

Specificity

Clone ELM1 is a rat monoclonal antibody that detects Nidogen-1 in murine species.

Immunogen

Purified protein from basement membranes of mouse Engelbreth-Holm-Swarm (EHS) tumor.

Application

Anti-Nidogen 1, clone ELM1, Cat. No. MAB1946-I, is a rat monoclonal antibodythat detects Nidogen-1 and has been tested for use in Immunocytochemistry, Immunohistochemistry, and Western Blotting.

Western Blotting Analysis: A representative lot detected Nidogen-1 in Western Blotting applications (Arends, F., et. al. (2015). PLoS One. 10(2):e0118090).

Immunocytochemistry Analysis: A representative lot detected Nidogen-1 in Immunocytochemistry applications (Kick, K., et. al. (2016). Arterioscler Thromb Vasc Biol. 36(12):2346-2357).

Immunohistochemistry Analysis: A representative lot detected Nidogen-1 in Immunohistochemistry applications (Chang, Y.T., et. al. (2016). J Clin Invest. 126(2):721-31).

Quality

Evaluated by Immunohistochemistry in mouse kidney.

Immunohistochemistry Analysis: A 1:50 dilution of this antibody detected Nidogen-1 in mouse kidney tissue.

Target description

136.54 kDa calculated.

Physical form

Format: Purified

Other Notes

Concentration: Please refer to lot specific datasheet.

Safety Information

• Storage Class Code: 12 - Non Combustible Liquids
• WGK: WGK 1
• Flash Point(F): Not applicable
• Flash Point(C): Not applicable