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CC325-1

Sigma-Aldrich

PhotoGel-RUT, Methacrylated Gelatin Hydrogel Kit

Photocrosslinked gelatin hydrogel (CC325 Kit Component 1)

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About This Item

UNSPSC Code:
12352207
NACRES:
NA.75

form

lyophilized powder

application(s)

cell analysis

General description

3D cell culture, including bioprinting, allows for the creation of more physiological cell models by allowing cells to simultaneously interact with integrins on all cell surfaces, resulting in the activation of specific signaling pathways not activated in traditional 2D cell culture methods. Hydrogels are water swollen polymers that allow for the culture of cells in 3-dimensions and can have profound effects on cellular development, differentiation, migration, and function. New areas of tissue engineering such as 3D bioprinting, have utilized UV photocrosslinked methacrylated hydrogel biomaterials (PEGMA, GelMA, HAMA and ColMA etc.) to encapsulate cells to make printable bioinks. The PhotoGel-RUT, Methacrylated Gelatin Hydrogel Kit (CC325: CC325-1, CC325-2) is based upon purified porcine gelatin methacrylate (GelMA), which when photocrosslinked provides a native-like 3D environment for cells. Gelatin derived from denatured collagen retains many natural cell binding motifs such as RGD and MMP sites. In addition to porcine gelatin methacrylate, the kit includes the photoinitiator ruthenium/sodium persulfate for users to easily fine tune their photocrosslinking experiments (i.e. altering hydrogel stiffness or gelling speeds). Gelatin methacrylate is produced from porcine, type A, 300 bloom gelatin. Gelatin macromers containing primary amino groups were reacted with methacrylic anhydride (MA) to add methacrylate pendant groups. The gelatin methacrylate achieves a degree of substitution of >70% for maximum crosslinking and range of stiffness.PhotoGel is a registered trademark of Advanced BioMatrix, Inc.

Application

Photocrosslinking is initiated using a 400-450 nm light source.

Packaging

1 Kit

Components

CC325-1 (Store at Room Temp): Ruthenium Photoinitiator, 1 X 100 mg (CS226450), Sodium Persulfate Photoinitiator, 1 X 500 mg (CS226449).
Note: The complete PhotoGel-RUT, Methacrylated Gelatin Hydrogel Kit (CC325) consists of CC325-1 and CC325-2 sold seperately.

Quality

Appearance: Lyophilized powderGelatin Content: ≥ 1 gramSterility (USP modified): No GrowthpH: 6.0-8.0Grafting Efficiency: ≥ 75%Cell Compatibility: >70% Cell Viability

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Signal Word

Danger

Hazard Classifications

Acute Tox. 4 Oral - Ox. Sol. 3 - Resp. Sens. 1 - Skin Irrit. 2 - Skin Sens. 1 - STOT SE 3

Target Organs

Respiratory system

Storage Class Code

5.1B - Oxidizing hazardous materials

Certificates of Analysis (COA)

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Articles

Photocrosslinkable Hydrogels for 3D Cell Culture and Tissue Engineering

Methacrylated collagen, hyaluronic acid, and gelatin (GelMA) hydrogels can be crosslinked with light and photoinitiators (Irgacure/LAP/Ruthenium), used as 3D cell culture scaffolds and bioinks for bioprinting.

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