ABN492

Anti-EPAC2 Antibody

from rabbit, purified by affinity chromatography

• Synonym(s): Rap guanine nuclear exchange factor 4, RAPGEF4, cAMPGEF-II, CGEF2, Exchange factor directly activated by cAMP 2
• UNSPSC Code: 12352203
• eCl@ss: 32160702
• NACRES: NA.41

Properties

• biological source: rabbit
• Quality Level: 100
• antibody form: affinity isolated antibody
• antibody product type: primary antibodies
• clone: polyclonal
• purified by: affinity chromatography
• species reactivity: mouse, rat
• species reactivity (predicted by homology): human (immunogen homology)
• technique(s): immunohistochemistry: suitable; western blot: suitable
• UniProt accession no.: Q8WZA2
• shipped in: wet ice
• target post-translational modification: unmodified
• Gene Information: human ... RAPGEF4(11069)

Description

General description

Exchange factor directly activated by cAMP 2 (EPAC2), also known as Rap guanine nuclear exchange factor 4 (RAPGEF4) and cAMP-regulated guanine nucleotide exchange factor II (cAMPGEF-II), belong to a family of cyclic adenosine monophosphate (cAMP) binding proteins that act as a guanine nucleotide exchange factor (GEF) for RAP1A, RAP1B, and RAP2A small GTPases that are activated through cAMP binding. EPAC2 signaling plays a role in numerous cellular processes such as integrin-mediated cell adhesion, muscle contraction, learning and memory, cell proliferation, exocytosis and inflammation. EPAC2 has been found to induce synapse remodeling and depression, with mutations in its gene being found in patients with autism. EPAC2 is predominantly expressed in brain and adrenal gland.

Immunogen

KLH-conjugated linear peptide corresponding to human EPAC2.

Application

Anti-EPAC2 Antibody is a highly specific rabbit polyclonal antibody, that targets Epac & has been tested in western blotting & IHC.

Immunohistochemistry Analysis: 2.5 µg/mL from a representative lot detected EPAC2 in mouse liver tissue.

Research Category
Neuroscience

Research Sub Category
Developmental Neuroscience

Quality

Evaluated by Western Blotting/Peptide Inhibition Assay in rat liver tissue lysate.

Western Blotting/Peptide Inhibition Assay Analysis: 1 µg/mL of this antibody detected EPAC2 in 15 µg of rat liver tissue lysate.

Target description

~129 kDa observed. Uncharacterized bands may appear in some lysate(s). Uniprot describes 3 isoforms at ~79 kDa, ~100 kDa, and ~116 kDa

Physical form

Antigen Affinity Purified

Purified rabbit polyclonal in buffer containing PBS with up to 0.1% sodium azide.

Storage and Stability

Stable for 1 year at 2-8°C from date of receipt.

Analysis Note

Control
Rat liver tissue lysate

Other Notes

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Safety Information

• Storage Class Code: 10 - Combustible liquids
• WGK: WGK 2
• Flash Point(F): Not applicable
• Flash Point(C): Not applicable