ABE472
Anti-WAC Antibody, NT
from rabbit, purified by affinity chromatography
Synonym(s):
WW domain-containing adapter protein with coiled-coil
Sign Into View Organizational & Contract Pricing
All Photos(2)
About This Item
UNSPSC Code:
12352203
eCl@ss:
32160702
NACRES:
NA.41
Recommended Products
biological source
rabbit
Quality Level
antibody form
affinity isolated antibody
antibody product type
primary antibodies
clone
polyclonal
purified by
affinity chromatography
species reactivity
human, mouse
technique(s)
ChIP: suitable
immunoprecipitation (IP): suitable
western blot: suitable
NCBI accession no.
UniProt accession no.
shipped in
wet ice
target post-translational modification
unmodified
Gene Information
human ... WAC(51322)
General description
WAC (WW domain-containing adapter protein with coiled-coil) functions as a linker between gene transcription and histone H2B monoubiquitination at ′Lys-120′ (H2BK120ub1). Wac interacts with the RNA polymerase II transcriptional machinery through its WW domain and with RNF20-RNF40 via its coiled-coil region. It has been shown to regulate H2BK120ub1, gene transcription, cell-cycle checkpoint activation in response to DNA damage, positively regulate amino acid starvation-induced autophagy and negatively regulate the ubiquitin proteasome pathway. In nuclear speckles it has been observed to colocalize with pre-mRNA processing complexes. This antibody recognizes the N-terminus of the WAC protein.
Immunogen
Recombinant protein corresponding to human WAC.
Application
Anti-WAC, N-terminus detects levels of WAC proteins & has been published & validated for use in WB, IP & ChIP.
Research Category
Epigenetics & Nuclear Function
Epigenetics & Nuclear Function
Research Sub Category
Chromatin Biology
Chromatin Biology
Western Blotting Analysis: 1 µg/mL from a representative lot detected WAC in 10 µg of HEK293 cell lysate.
Immunoprecipitation Analysis: A representative lot from an independent laboratory detected WAC, N-terminus in 293T cell lysates stably transfected with SFB-RNF20 and in HCT116 cell lysates transfected with siRNA negative control (Zhang, F. and Yu, X. (2011). Mol Cell. 41(4):384-397.).
Chromatin Immunoprecipitation: A representative lot from an independent laboratory detected WAC, N-terminus in HCT116 cell lysates transfected with siRNA negative control, but demonstrated a loss of signal in HCT166 cell lysates transfected with siRNF20 (Zhang, F. and Yu, X. (2011). Mol Cell. 41(4):384-397.).
Immunoprecipitation Analysis: A representative lot from an independent laboratory detected WAC, N-terminus in 293T cell lysates stably transfected with SFB-RNF20 and in HCT116 cell lysates transfected with siRNA negative control (Zhang, F. and Yu, X. (2011). Mol Cell. 41(4):384-397.).
Chromatin Immunoprecipitation: A representative lot from an independent laboratory detected WAC, N-terminus in HCT116 cell lysates transfected with siRNA negative control, but demonstrated a loss of signal in HCT166 cell lysates transfected with siRNF20 (Zhang, F. and Yu, X. (2011). Mol Cell. 41(4):384-397.).
Quality
Evaluated by Western Blotting in K562 cell lysate.
Western Blotting Analysis: 1 µg/mL of this antibody detected WAC, N-terminus in 10 µg of K562 cell lysate.
Western Blotting Analysis: 1 µg/mL of this antibody detected WAC, N-terminus in 10 µg of K562 cell lysate.
Target description
~80 kDa observed. Multiple isoforms are known to exist at ~70 kDa, ~65 kDa, ~60 kDa, and ~47 kDa Uncharacterized bands may be observed at ~52 kDa, ~75 kDa, and ~270 kDa in some cell lysates.
Physical form
Affinity purified
Purified rabbit polyclonal in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.
Storage and Stability
Stable for 1 year at 2-8°C from date of receipt.
Analysis Note
Control
K562 cell lysate
K562 cell lysate
Other Notes
Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.
Disclaimer
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Not finding the right product?
Try our Product Selector Tool.
Storage Class Code
12 - Non Combustible Liquids
WGK
WGK 1
Flash Point(F)
Not applicable
Flash Point(C)
Not applicable
Certificates of Analysis (COA)
Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.
Already Own This Product?
Find documentation for the products that you have recently purchased in the Document Library.
Feng Zhang et al.
Molecular cell, 41(4), 384-397 (2011-02-19)
Histone H2B ubiquitination plays an important role in regulating chromatin organization during gene transcription. It has been shown that RNF20/40 regulates H2B ubiquitination. Here, using protein affinity purification, we have identified WAC as a functional partner of RNF20/40. Depletion of
Our team of scientists has experience in all areas of research including Life Science, Material Science, Chemical Synthesis, Chromatography, Analytical and many others.
Contact Technical Service