AB5320A4
Anti-NG2, Alexa Fluor™488 Conjugate Antibody
from rabbit, ALEXA FLUOR™ 488
Synonym(s):
Chondroitin sulfate proteoglycan 4, Chondroitin sulfate proteoglycan NG2, Melanoma chondroitin sulfate proteoglycan, Melanoma-associated chondroitin sulfate proteoglycan
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All Photos(2)
About This Item
UNSPSC Code:
12352203
eCl@ss:
32160702
NACRES:
NA.41
Recommended Products
biological source
rabbit
Quality Level
conjugate
ALEXA FLUOR™ 488
antibody form
purified antibody
antibody product type
primary antibodies
clone
polyclonal
species reactivity
rat
species reactivity (predicted by homology)
monkey (based on 100% sequence homology), human (based on 100% sequence homology), mouse (based on 100% sequence homology)
technique(s)
immunocytochemistry: suitable
immunohistochemistry: suitable
NCBI accession no.
UniProt accession no.
shipped in
wet ice
target post-translational modification
unmodified
Gene Information
human ... CSPG4(1464)
mouse ... Cspg4(121021)
rat ... Cspg4(81651)
rhesus monkey ... Cspg4(713086)
General description
NG2 is a high molecular weight, integral membrane chondroitin sulfate proteoglycan. It is found on the surfaces of an unusual class of glial cells within the developing and mature central nervous system that have the properties of oligodendrocyte precursor cells (i.e., O-2A progenitor cells). NG2 is also found on the surfaces of chondroblasts, proliferating capillary endothelial cells, some human melanoma cell lines, and on leukemic blasts in childhood acute lymphoblastic leukemia. The NG2 proteoglycan is likely to play a role in regulation of cell motility, axon outgrowth and the cellular responses to platelet-derived growth factor.
Specificity
This antibody identifies the intact proteoglycan and core protein by WB and ELISA. When oligodendrocyte precursor cells (i.e. O-2A progenitor cells) are stained alive, the stain appears as clusters on the cell surface. This antibody does not stain differentiated oligodendrocytes well.
Immunogen
Immunoaffinity purified NG2 Chondroitin Sulfate Proteoglycan from rat
Application
Anti-NG2, Alexa Fluor™488 Conjugate Antibody detects level of NG2 & has been published & validated for use in ICC & IHC.
Immunohistochemistry Analysis: A 1:100 dilution from a representative lot detected NG2 in adult rat brain tissue.
Research Category
Neuroscience
Neuroscience
Research Sub Category
Neurodegenerative Diseases
Neurodegenerative Diseases
Quality
Evaluated by Immunocytochemistry in rat oligodendrocyte precursor cells.
Immunocytochemistry Analysis: A 1:100 dilution of this antibody detected NG2 in rat oligodendrocyte precursor cells. Performed without Triton.
Immunocytochemistry Analysis: A 1:100 dilution of this antibody detected NG2 in rat oligodendrocyte precursor cells. Performed without Triton.
Target description
The unconjugated parent antibody (Cat. No. AB5320) has an observed molecular weight at ~270-300 kDa
Physical form
Protein A adsorption
Purified rabbit polyclonal conjugated to Alexa Fluor™ 488 in PBS with 0.1% sodium azide and 15 mg/mL BSA.
Storage and Stability
Maintain refrigerated at 2-8 °C protected from light in undiluted aliquots for up to 6 months from date of receipt.
Analysis Note
Control
Rat or Human oligodendrocyte precursor cells
Rat or Human oligodendrocyte precursor cells
Legal Information
ALEXA FLUOR is a trademark of Life Technologies
Disclaimer
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
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Storage Class Code
10 - Combustible liquids
WGK
WGK 2
Flash Point(F)
Not applicable
Flash Point(C)
Not applicable
Certificates of Analysis (COA)
Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.
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