AB3560P
Anti-Sodium Antibody, Potassium, Chloride Cotransporter 1
Chemicon®, from rabbit
Synonym(s):
Basolateral Na-K-Cl symporter, Bumetanide-sensitive sodium-(potassium)-chloride cotransporter 1, solute carrier family 12 sodium/potassium/chloride transporters), member 2
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About This Item
UNSPSC Code:
12352203
eCl@ss:
32160702
NACRES:
NA.41
biological source
rabbit
Quality Level
antibody form
affinity purified immunoglobulin
antibody product type
primary antibodies
clone
polyclonal
purified by
affinity chromatography
species reactivity
human, bovine, mouse, rat
manufacturer/tradename
Chemicon®
technique(s)
ELISA: suitable
immunohistochemistry: suitable
western blot: suitable
NCBI accession no.
UniProt accession no.
shipped in
dry ice
target post-translational modification
unmodified
Gene Information
human ... SLC12A2(6558)
General description
The Na+/K+/Cl- Cotransporter isoform 1 (NKCC1) is involved in the transport of sodium, potassium, and chloride ions across membranes. NKCC1 mediates sodium and chloride reabsorption and plays an important role in cell volume regulation. NKCC1 is also involved in the control of normal cell proliferation, while its overexpression results in apparent cell transformation, in a manner similar to some protooncogenes (Panet, 1999). NKCC1 is inhibited by bumetanide and is also referred to as the bumetanide-sensitive sodium-(potassium)-chloride cotransporter 1. Inhibition of NKCC1 activity by bumetanide decreases the high-[K+]o-mediated uptake of Cl , blocks astrocyte swelling, and leads to cell shrinkage under isotonic conditions in cells such as retinal pigment epithelia, ventricular myocytes, and vascular endothelial cells (Russell, 2000; Su, 2002). NKCC1 could contribute to an overload of intracellular Na+, K+, and Cl- and cell swelling in conditions such as cerebral ischemia (Yan, 2001). NKCC1 is expressed in multiple tissues with the amount of mRNA expression varying as much as thirty-fold. It is highly expressed in neurons and astrocytes through-out the brain and has been implicated in ischemic necrotic cell death.
Specificity
Expected to cross-react with human and mouse. The immunogen sequence is 95% conserved in mouse, 86% in human, and 82% in bovine.
Recognizes rat Sodium, Potassium, Chloride Cotransporter 1 (NKCC1, BSC2, CCC1). The immunogen shows no significant sequence homology with other CCC.
Immunogen
A 22 amino acid peptide sequence near the C-terminus of rat NKCC1. (Moore-Hoon et al. 1998; Delpire et al. 1994; Payne et al. 1995; Isering et al. 1997; Mount et al. 1998).
Application
Detect Sodium using this Anti-Sodium Antibody, Potassium, Chloride Cotransporter 1 validated for use in ELISA, IH & WB.
Immunohistochemistry:
It is recommended that the antibody be tried at 2-20 µg/mL on paraformaldehyde fixed tissue.
ELISA:
0.5-1.0 µg/mL of a previous lot was used with 1 µg/mL control peptide per well.
Optimal working dilutions must be determined by the end user.
It is recommended that the antibody be tried at 2-20 µg/mL on paraformaldehyde fixed tissue.
ELISA:
0.5-1.0 µg/mL of a previous lot was used with 1 µg/mL control peptide per well.
Optimal working dilutions must be determined by the end user.
Research Category
Neuroscience
Neuroscience
Research Sub Category
Ion Channels & Transporters
Ion Channels & Transporters
Quality
Evaluated by Western Blot on Rat Spinal Cord lysates.
Western Blot Analysis:
1:500 dilution detected NA+K+CL- COTRANS 1 on 10 µg of Rat Spinal Cord lysates.
Western Blot Analysis:
1:500 dilution detected NA+K+CL- COTRANS 1 on 10 µg of Rat Spinal Cord lysates.
Target description
170 kDa
Physical form
Affinity purified rabbit polyclonal in buffer containing PBS with 0.1% BSA.
ImmunoAffinity Purified
Storage and Stability
Stable for 6 months at -20°C in undiluted aliquots from date of receipt.
Handling Recommendations: Upon first thaw, and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance.
Handling Recommendations: Upon first thaw, and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance.
Analysis Note
Control
Spinal cord, colon.
Spinal cord, colon.
Other Notes
Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.
Legal Information
CHEMICON is a registered trademark of Merck KGaA, Darmstadt, Germany
Disclaimer
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
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Storage Class Code
12 - Non Combustible Liquids
WGK
WGK 2
Flash Point(F)
Not applicable
Flash Point(C)
Not applicable
Certificates of Analysis (COA)
Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.
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Mari A Virtanen et al.
Cells, 9(12) (2020-12-10)
Ionotropic GABA transmission is mediated by anion (mainly Cl-)-permeable GABAA receptors (GABAARs). In immature neurons, GABA exerts depolarizing and sometimes functionally excitatory actions, based on active uptake of Cl- by the Na-K-2Cl cotransporter NKCC1. While functional evidence firmly shows NKCC1-mediated
Jichao Chen et al.
Developmental cell, 13(3), 325-337 (2007-09-04)
In the mammalian inner ear, endolymph is produced and resorbed by a complex series of epithelia. We show here that estrogen-related receptor beta (ERR-beta; NR3B2), an orphan nuclear receptor, is specifically expressed in and controls the development of the endolymph-producing
Endolymphatic Na? and K? concentrations during cochlear growth and enlargement in mice lacking Slc26a4/pendrin.
Li, X; Zhou, F; Marcus, DC; Wangemann, P
Testing null
Cellular distribution of NKCC2 in the gastric mucosa and its response to short-term osmotic shock.
T Ji,S Liu,L F Zheng,Q Wang,Z F Dou,Y Zhang,J X Zhu
Cell and Tissue Research null
Y Zhu et al.
Neuroscience, 220, 330-340 (2012-06-26)
Persistent inflammation is associated with a shift in spinal GABA(A) signaling from inhibition to excitation such that GABA(A)-receptor activation contributes to inflammatory hyperalgesia. We tested the hypothesis that the primary afferent is the site of the persistent inflammation-induced shift in
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