1.25085

Fractogel^® EMD TMAE Hicap (M)

RoboColumn Fractogel^® TMAE Hicap (M), volume 0.2 mL, prepacked column

• Synonym(s): RoboColumn Fractogel^® TMAE Hicap (M), 0.2 ml
• UNSPSC Code: 41115711

Properties

• ligand: (Trimethylammoniumethyl)
• Quality Level: 100
• material: polypropylene housing
• product line: Fractogel^®
• form: prepacked column
• parameter: ≤8 bar pressure; ~180 cm/hr max. flow rate
• column I.D. × L: 5 mm × 10 mm
• volume: 0.2 mL
• matrix active group: Methacrylate
• mean particle size: 40-90 μm
• capacity: ~180 mg, BSA binding capacity
• application(s): mAb; recombinant protein; vaccine development
• separation technique: anion exchange
• storage temp.: room temp

Description

General description

5 x 10 mm

Features and Benefits

RoboColumn Fractogel^® TMAE Hicap (M), 0.2 ml enables users to:

• Efficiently capture target protein and remove viruses, DNA, and endotoxins with its tentacle technology resulting in better production yields and high throughput
• Reduce operating costs and resin replacement with enduring durability - allowing for multiple cycles of column regeneration and sanitization
• Compatibility with 2.5 % (v/v) aqueous benzyl alcohol containing 150 mM NaCl storage solution

Due to the titration behavior of Fractogel^® EMD TMAE Hicap (M) the ion exchange capacity can be used from pH 2 to 12. The separation of proteins is based on reversible electrostatic interactions between the negatively charged regions of the proteins′ surface and the support. Proteins are retained efficiently on Fractogel^® EMD TMAE Hicap (M) when the pH of the buffer is about 1 unit above their isoelectric points (pl).

The strength of the binding depends on the following:

• the buffer system
• pH value of the buffer which determines the surface charge of the protein
• the concentration of the counter ions
• the charge density on the support (protein binding capacity)

Legal Information

FRACTOGEL is a registered trademark of Merck KGaA, Darmstadt, Germany