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1.02449

Millipore

Readybag® Half FRASER broth with supplements

pkg of 60 pieces, according to FDA (BAM), ISO 11290, for Listeria spp.

Synonym(s):

Demi FRASER broth with supplements

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About This Item

UNSPSC Code:
41106213
NACRES:
NA.74

Agency

APHA
ISO 11290

Quality Level

200

reg. compliance

according to FDA (BAM)

sterility

sterile; γ-irradiated

form

medium granules (dehydrated (DCM))

packaging

pkg of 60 pieces
pkg of

manufacturer/tradename

ReadyBag®

technique(s)

microbiological culture: suitable

pH

7.0-7.4 (25 °C, 55 g/L in H2O)

solubility

55 g/L

application(s)

food and beverages
microbiology
pathogen testing

storage temp.

15-25°C

suitability

Listeria spp. (spp.)

Related Categories

General description

Readybag® Half FRASER broth with supplements come in pre-weighed and gamma-irradiated to speed up and simplify food pathogen testing routines. This helps to eliminate all the media preparation steps from the workflow. The Readybag® single-use pouches remove the necessity of weighing, autoclaving, supplement handling, and extra lab space or equipment. It only requires the addition of sterile water before incubation.
The high nutrient content and the large buffer capacity of the Readybag® Half FRASER broth (with supplements) create optimum growth conditions for Listeria spp. Lithium chloride, nalidixic acid, and acriflavine hydrochloride inhibit the growth of accompanying bacteria. Esculin and ammonium iron (III) citrate forms a black complex of esculetin with iron (III) ions and facilitates the detection of β-D-glucosidase activity. But this reaction is not exclusive to Listeria spp. Hence, every primary and secondary enrichment in Fraser broth must be sub-cultured on selective plating media.

Application

Readybag® Half FRASER broth (with supplements) is suitable for the primary selective enrichment of Listeria spp. from food and animal feed, environmental samples, and other materials.

Packaging

Each sachet contains 12.5 g of granulated Half Fraser broth to which 225 mL of sterile water is added

Legal Information

READYBAG is a registered trademark of Merck KGaA, Darmstadt, Germany

Storage Class Code

11 - Combustible Solids

WGK

WGK 3

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

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Articles

Detection of Listeria monocytogenes in Food and Environmental Samples

Detection of Listeria monocytogenes in food and environmental samples according to ISO 11290-1, FDA-BAM and USDA-FSIS

Protocols

Listeria: a Survivor

In recent years it has been recognized that Listeria is an important public health problem. The disease affects primarily people of advanced age, pregnant women, newborns, and adults with weakened immune systems.

Our team of scientists has experience in all areas of research including Life Science, Material Science, Chemical Synthesis, Chromatography, Analytical and many others.

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