1.00070
mCCD (Modified charcoal cefoperazone deoxycholate) agar (base)
acc. ISO 10272 GranuCult®
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About This Item
UNSPSC Code:
41106213
NACRES:
NA.74
Agency
ISO
Quality Level
form
powder (dehydrated (DCM))
manufacturer/tradename
GranuCult®
technique(s)
microbiological culture: suitable
pH
7.2-7.6 (25 °C, 44.5 g/L in H2O)
solubility
45.5 g/L
application(s)
food and beverages
microbiology
pathogen testing
storage temp.
15-25°C
suitability
Campylobacter spp.
General description
GranuCult is our new brand name for our existing granulated dehydrated culture media which apply to new EN ISO 11133:2014 and various other ISO standards and FDA-BAM / USDA-FSIS methods for testing of food and water samples. All these media are currently being updated to be fully compliant to the latest version of these standards and regulations with respect to composition and quality control and will be available shortly.
Analysis Note
Appearance (colour): black
pH-value (25 °C): 7.2 - 7.6
Typical composition (g/litre): Peptones 20.0; Casein hydrolysate 30.; Activated charcoal 4.0; Sodium deoxycholate 1.0; Sodium pyruvate 0.25; Ferrous sulfate 0.25; Agar-agar 12.0.
Growth promotion test in accordance with ISO 11133.
Base tested with supplement Art. 1.00071.
Growth (Campylobacter coli ATCC 43478 (WDCM 00004)): good
Growth (Campylobacter jejuni ATCC 33291 (WDCM 00005)): good
Growth (Campylobacter jejuni ATCC 29428 (WDCM 00156)): good
Growth (Staphylococcus aureus ATCC 25923 (WDCM 00034)): no growth
Growth (Escherichia coli ATCC 25922 (WDCM 00013)): inhibited growth
Growth (Escherichia coli ATCC 8739 (WDCM 00012)): inhibited growth
Incubation: 44 hrs. 41.5 °C with Anaerocult C. E.coliand Staph.aur. aerobic.
Strains tested with three-loop-streak.
pH-value (25 °C): 7.2 - 7.6
Typical composition (g/litre): Peptones 20.0; Casein hydrolysate 30.; Activated charcoal 4.0; Sodium deoxycholate 1.0; Sodium pyruvate 0.25; Ferrous sulfate 0.25; Agar-agar 12.0.
Growth promotion test in accordance with ISO 11133.
Base tested with supplement Art. 1.00071.
Growth (Campylobacter coli ATCC 43478 (WDCM 00004)): good
Growth (Campylobacter jejuni ATCC 33291 (WDCM 00005)): good
Growth (Campylobacter jejuni ATCC 29428 (WDCM 00156)): good
Growth (Staphylococcus aureus ATCC 25923 (WDCM 00034)): no growth
Growth (Escherichia coli ATCC 25922 (WDCM 00013)): inhibited growth
Growth (Escherichia coli ATCC 8739 (WDCM 00012)): inhibited growth
Incubation: 44 hrs. 41.5 °C with Anaerocult C. E.coliand Staph.aur. aerobic.
Strains tested with three-loop-streak.
Footnote
We offer two media types: the superior granulated GranuCult® and the cost-efficient powdered NutriSelect® culture media, depending on your needs.
The designations basic, plus, or prime are added to indicate the quality control level, from basic quality control to standard QC plus to prime for full regulatory compliance.
The designations basic, plus, or prime are added to indicate the quality control level, from basic quality control to standard QC plus to prime for full regulatory compliance.
Legal Information
GRANUCULT is a registered trademark of Merck KGaA, Darmstadt, Germany
NutriSelect is a registered trademark of Merck KGaA, Darmstadt, Germany
Storage Class Code
11 - Combustible Solids
WGK
WGK 3
Certificates of Analysis (COA)
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