06-680-AF555

Anti-Androgen Receptor Antibody, Alexa Fluor^™ 555 Conjugate

from rabbit, ALEXA FLUOR^™ 555

• Synonym(s): Androgen receptor, Dihydrotestosterone receptor, Nuclear receptor subfamily 3 group C member 4
• UNSPSC Code: 12352203
• eCl@ss: 32160702

Properties

• biological source: rabbit
• Quality Level: 100
• conjugate: ALEXA FLUOR^™ 555
• antibody form: affinity isolated antibody
• antibody product type: primary antibodies
• clone: polyclonal
• species reactivity: human, rat
• species reactivity (predicted by homology): mouse (based on 100% sequence homology), canine (based on 100% sequence homology), horse (based on 100% sequence homology), bovine (based on 100% sequence homology)
• technique(s): immunocytochemistry: suitable
• NCBI accession no.: NP_036634
• UniProt accession no.: P15207
• target post-translational modification: unmodified
• Gene Information: bovine ... Ar(280675) ; dog ... Ar(403588) ; human ... AR(367) ; mouse ... Ar(11835) ; rat ... Ar(24208)

Description

General description

Androgen receptor (UniProt P15207; also known as Dihydrotestosterone receptor, Nuclear receptor subfamily 3 group C member 4) is encoded by the Ar (also known as Andr, Nr3c4, Tfm) gene (Gene ID 24208) in rat species. Androgen receptor (AR) is a hormone-activated transcription regulator that plays important roles in normal prostate development and function, as well as in malignant prostate transformation. The binding of an androgenic hormone (testosterone or dihydrotestosterone) induces a conformational change in AR, resulting in its dissociation from heat schock proteins and subsequent translocation from the cytosol to nucleus, where it dimerizes and mediates either transcription activation or repression in a target gene-specific manner. The androgen insensitivity syndrome (AIS) or testicular feminization (TFM) is caused by a mutation of the androgen receptor gene located on the X chromosome.

Immunogen

Epitope: N-terminal modulating region.

KLH-conjugated linear peptide corresponding to the N-terminal sequence of rat androgen receptor.

Application

Research Category
Epigenetics & Nuclear Function

Research Sub Category
Hormones & Receptors

The unconjugated antibody (Cat. No. 06-680) is shown to be suitable also for chromatin immunoprecipitation (ChIP), ChIP-seq, Western blotting, immunoprecipitation, flow cytometry, immunohistochemistry applications.

This Anti-Androgen Receptor Antibody, Alexa Fluor^™ 555 Conjugate is validated for use in Immunocytochemistry for the detection of Androgen Receptor.

Quality

Evaluated by Immunocytochemistry in T47D cells.

Immunocytochemistry Analysis: A 1:100 dilution of this antibody detected androgen receptor in T47D human breast cancer cells.

Target description

~110 kDa observed.

Physical form

Affinity purified.

Purified rabbit polyclonal antibody conjugate in PBS with 15 mg/mL BSA and 0.1 % sodium azide.

Storage and Stability

Stable for 1 year at 2-8°C from date of receipt.

Other Notes

Concentration: Please refer to lot specific datasheet.

Legal Information

ALEXA FLUOR is a trademark of Life Technologies

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Safety Information

• Storage Class Code: 12 - Non Combustible Liquids
• WGK: WGK 2
• Flash Point(F): Not applicable
• Flash Point(C): Not applicable