05-1085

Anti-IRS1 Antibody, clone 4.2.2

clone 4.2.2, from mouse

• Synonym(s): insulin receptor substrate 1
• UNSPSC Code: 12352203
• eCl@ss: 32160702
• NACRES: NA.41

Properties

• biological source: mouse
• Quality Level: 100
• antibody form: purified antibody
• antibody product type: primary antibodies
• clone: 4.2.2, monoclonal
• species reactivity: pig, canine, human, mouse, rat, monkey, bovine
• technique(s): immunocytochemistry: suitable; immunoprecipitation (IP): suitable; western blot: suitable
• isotype: IgG2aκ
• NCBI accession no.: NP_005535.1
• UniProt accession no.: P35568
• shipped in: wet ice
• target post-translational modification: unmodified
• Gene Information: bovine ... Irs1(538598) ; dog ... Irs1(486148) ; human ... IRS1(3667) ; mouse ... Irs1(16367) ; pig ... Irs1(100512686) ; rat ... Irs1(25467) ; rhesus monkey ... Irs1(707870)

Description

General description

IRS1 (Insulin Receptor Substrate 1) transmits insulin signals via metabolic and mitogenic pathways. IRS1 is heavily phosphorylated on both serine and tyrosine residues. These phosphorylated tyrosines enable IRS to act as a docking protein that binds SH2 domains of such proteins as PI3 Kinase (phosphatidylinositol 3-kinase) and GRB2, resulting in activation. Over expression and phosphorylation of serine is associated with insulin resistance and breast cancer. Some of the more notable phosphorylation sites are Ser302 that is phosphorylated following insulin stimulation. Ser307, phosphorylated by JNK and IKK, is a key regulatory site that appears to disrupt the IRS1/IR interaction and inhibits insulin-mediated activation of the PI3 kinase and MAPK pathways, and Ser636/639 that is known to be phosphorylated by p70S6K downstream of mTOR and acts as a negative feedback loop.

Specificity

Predicted to cross-react with many other species based on 100% sequence homology with immunogen.

This antibody recognizes IRS1.

Immunogen

Synthetic peptide corresponding to amino acids 431-446 of mouse IRS1.

Application

This Anti-IRS1 Antibody, clone 4.2.2 is validated for use in WB, IP, IC for the detection of IRS1.

Quality

Evaluated by western blot on IR/IRS1 transfected CHO +/- Insulin lysate.

Western Blot Analysis:
1:1,000 dilution of this antibody was used to detect IRS1 in IRS/IR transfected CHO -/+ Calyculin A/ Okadaic Acid-treated cell lysate.

Target description

~185 kDa

Physical form

Format: Purified

Purified mouse monoclonal antibody in buffer containing 0.1 M Tris-Glycine (pH 7.4),150 mM NaCl with 0.05% sodium azide.

Other Notes

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

Safety Information

• Storage Class Code: 12 - Non Combustible Liquids
• WGK: WGK 1
• Flash Point(F): Not applicable
• Flash Point(C): Not applicable