HPLC Analysis of Acrylamide and Acrylic Acid on Discovery^® HS F5

column

Discovery HS F5, 15 cm × 4.6 mm I.D., 5 μm particles (567516-U)

mobile phase

0.05% formic acid

flow rate

1.0 mL/min

column temp.

ambient

detector

UV, 206 nm

injection

1 μL

sample

1.0 mg/mL each in mobile phase