HPLC Analysis of Folinic Acid Enantiomers on Astec® CHIROBIOTIC® T (RP mode)
CONDITIONS
column
CHIROBIOTIC T, 25 cm x 4.6 mm I.D., 5 μm particles (12024AST)
mobile phase
[A] 1% triethylamine acetate, pH 4.5; [B] ethanol; (50:50, A:B)
flow rate
1 mL/min
column temp.
20 °C
detector
UV, 254 nm
injection
5 μL
sample
1.25 mg/mL in methanol
说明
法律資訊
Astec is a registered trademark of Merck KGaA, Darmstadt, Germany
CHIROBIOTIC is a registered trademark of Sigma-Aldrich Co. LLC