HPLC Analysis of Carboxymethylated Cytochrome c (Chicken) Tryptic Digest on Discovery^® BIO Wide Pore C18

column

Discovery BIO Wide Pore C18, 15 cm × 2.1 mm I.D., 3 μm particles (567202-U)

mobile phase

[A] water/0.1% TFA; [B] (water/0.1%TFA):(acetonitrile/0.1% TFA); 50:50

flow rate

0.20 mL/min

column temp.

35 °C

detector

UV, 215 nm

injection

5 μL