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Merck

HPLC Analysis of an Energy Drink on Ascentis® Express HILIC (UV Detection)

HPLC Analysis of an Energy Drink on Ascentis® Express HILIC (UV Detection) application for HPLC

材料

分析柱

产品编号
说明
价格

53970-U

Ascentis® Express HILIC, 2.7 μm HPLC色谱柱

2.7 μm particle size, L × I.D. 10 cm × 3 mm

標準

产品编号
说明
价格

C1778

咖啡因

Sigma Reference Standard, vial of 250 mg

S1626

山梨酸

99.0-101.0% anhydrous basis

47862

盐酸吡哆素 (B6)

analytical standard

47865-U

尼克酰胺（烟酰胺）

analytical standard

47861

核黄素 (B2)

analytical standard

8S61336

SS苯甲酸

2000 μg/mL in dichloromethane, analytical standard

CONDITIONS

column

Ascentis Express HILlC, 10 cm x 3.0 mm I.D., 2.7 μm particles (53970-U)

mobile phase

[A] 100 mM ammonium acetate, pH 5.0 with acetic acid; [B] water; [C] acetonitrile; (09:01:90, A:B:C)

flow rate

0.6 mL/min

pressure

812 psi (56 bar)

column temp.

35 °C

detector

UV, 254 nm

injection

2 μL

sample

Dilute 1:9 in acetonitrile

说明

分析報告

This application demonstrates the suitability of Ascentis Express HILIC for the efficient separation of common drink additives used in caffeinated energy drinks. The analysis was performed with UVand ELSD detectors in series, this chromatogram being from the UV.

法律資訊

Ascentis is a registered trademark of Merck KGaA, Darmstadt, Germany

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