GC Analysis of Proline Methyl Ester Enantiomers (N-Acetyl Derivatives) on Astec^® CHIRALDEX^™ G-TA

column

Astec CHIRALDEX G-TA, 30 m x 0.25 mm I.D., 0.12 μm (73033AST)

oven

155 °C

inj. temp.

250 °C

detector

FID, 250 °C

carrier gas

helium, 30 psi

injection

1 μL, 80:1 split

sample

3 mg/mL racemate in methanol
peak 1: D-proline methyl ester (N-acetyl derivative)
peak 2: L-proline methyl ester (N-acetyl derivative