GC Analysis of Cocaine in Urine on a 100% Methyl Column after SPME using 100 μm PDMS Fiber
CONDITIONS
sample/matrix
0.5 mL urine (250 ng each analyte, 20 μL 2.5% NaF) in 1 mL vial
SPME fiber
polydimethylsiloxane, 100 μm (57300-U)
extraction
immersion, 30 min
desorption process
3 min, 240 °C
column
100% methyl polysiloxane, 30 m × 0.32 mm I.D., 0.25 μm (Supelco equivalent, Equity-1, 28055-U)
oven
120 °C to 280 °C at 10 °C/min
inj. temp.
splitless (splitter opened after 1 min), 240 °C
detector
NPD, 280 °C
carrier gas
helium, 3 mL/min